Studies on the respiration rate of free and immobilized cells of Cephalosporium acremonium in cephalosporin C production

Página do item simplificado
dc.contributor.authorAraujo, MLGC
dc.contributor.authorGiordano, R. C.
dc.contributor.authorHokka, C. O.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2014-05-20T15:29:54Z
dc.date.available2014-05-20T15:29:54Z
dc.date.issued1999-06-05
dc.description.abstractBioprocesses using filamentous fungi immobilized in inert supports present many advantages when compared to conventional free cell processes. However, assessment of the real advantages of the unconventional process demands a rigorous study of the limitations to diffusional mass transfer of the reagents, especially concerning oxygen. In this work, a comparative study was carried out on the cephalosporin C production process in defined medium containing glucose and sucrose as main carbon and energy sources, by free and immobilized cells of Cephalosporium acremonium ATCC 48272 in calcium alginate gel beads containing alumina. The effective diffusivity of oxygen through the gel beads and the effectiveness factors related to the respiration rate of the microorganism were determined experimentally. By applying Monod kinetics, the respiration kinetics parameters were experimentally determined in independent experiments in a complete production medium. The effectiveness factor experimental values presented good agreement with the theoretical values of the approximated zero-order effectiveness factor, considering the dead core model. Furthermore, experimental results obtained with immobilized cells in a 1.7-L tower bioreactor were compared with those obtained in 5-L conventional fermenter with free cells. It could be concluded that it is possible to attain rather high production rates working with relatively large diameter gel beads (ca. 2.5 mm) and sucrose consumption-based productivity was remarkably higher with immobilized cells, i.e., 0.33 gCPC/kg sucrose/h against 0.24 gCPC/kg sucrose/h in the aerated stirred tank bioreactor process. (C) 1999 John Wiley & Sons, Inc.en
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Dept Engn Quim, Sao Carlos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14801970 Araraquara, SP, Brazil
dc.format.extent593-600
dc.identifierhttp://dx.doi.org/10.1002/(SICI)1097-0290(19990605)63:5<593
dc.identifier.citationBiotechnology and Bioengineering. New York: John Wiley & Sons Inc., v. 63, n. 5, p. 593-600, 1999.
dc.identifier.doi10.1002/(SICI)1097-0290(19990605)63:5<593
dc.identifier.issn0006-3592
dc.identifier.urihttp://hdl.handle.net/11449/39370
dc.identifier.wosWOS:000079918700009
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBiotechnology and Bioengineering
dc.relation.ispartofjcr3.952
dc.relation.ispartofsjr1,372
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcephalosporin C productionpt
dc.subjectimmobilized cellspt
dc.subjectreaction-diffusion modelpt
dc.subjectCa-alginate oxygen diffusivitypt
dc.subjecteffectiveness factorpt
dc.titleStudies on the respiration rate of free and immobilized cells of Cephalosporium acremonium in cephalosporin C productionen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Bioquímica e Tecnologia - IQ