SAXS Studies of the Endoglucanase Cel12A from Gloeophyllum trabeum Show Its Monomeric Structure and Reveal the Influence of Temperature on the Structural Stability of the Enzyme
dc.contributor.author | Miotto, Lis S. | |
dc.contributor.author | Reis, Caio V. dos | |
dc.contributor.author | Neto, Mario de Oliveira [UNESP] | |
dc.contributor.author | Polikarpov, Igor | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2015-03-18T15:56:21Z | |
dc.date.available | 2015-03-18T15:56:21Z | |
dc.date.issued | 2014-07-01 | |
dc.description.abstract | Endoglucanases are key enzymes applied to the conversion of biomass aiming for second generation biofuel production. In the present study we obtained the small angle X-ray scattering (SAXS) structure of the G. trabeum endo-1,4-beta-glucanase Cel12A and investigated the influence of an important parameter, temperature, on both secondary and tertiary structure of the enzyme and its activity. The CD analysis for GtCel12A revealed that changes in the CD spectra starts at 55 degrees C and the T-m calculated from the experimental CD sigmoid curve using the Boltzmann function was 60.2 +/- 0.6 degrees C. SAXS data showed that GtCel12A forms monomers in solution and has an elongated form with a maximum diameter of 60 +/- 5 angstrom and a gyration radius of 19.4 +/- 0.1 angstrom as calculated from the distance distribution function. Kratky analysis revealed that 60 degrees C is the critical temperature above which we observed clear indications of denaturation. Our results showed the influence of temperature on the stability and activity of enzymes and revealed novel structural features of GtCel12A. | en |
dc.description.affiliation | Univ Sao Paulo, Inst Fis Sao Carlos, Grp Biotecnol Mol, BR-13566590 Sao Carlos, SP, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Fis & Biofis, BR-18618970 Botucatu, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Inst Biociencias Botucatu, Dept Fis & Biofis, BR-18618970 Botucatu, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: 09/08233-9 | |
dc.format.extent | 5202-5211 | |
dc.identifier | http://dx.doi.org/10.3390/ma7075202 | |
dc.identifier.citation | Materials. Basel: Mdpi Ag, v. 7, n. 7, p. 5202-5211, 2014. | |
dc.identifier.doi | 10.3390/ma7075202 | |
dc.identifier.file | WOS000339989700015.pdf | |
dc.identifier.issn | 1996-1944 | |
dc.identifier.lattes | 8213371495151651 | |
dc.identifier.uri | http://hdl.handle.net/11449/117516 | |
dc.identifier.wos | WOS:000339989700015 | |
dc.language.iso | eng | |
dc.publisher | Mdpi Ag | |
dc.relation.ispartof | Materials | |
dc.relation.ispartofjcr | 2.467 | |
dc.relation.ispartofsjr | 0,732 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | endoglucanase | en |
dc.subject | Gloeophyllum trabeum | en |
dc.subject | biophysics | en |
dc.subject | circular dichroism | en |
dc.subject | small angle X-ray scattering (SAXS) | en |
dc.subject | Kratky analysis | en |
dc.title | SAXS Studies of the Endoglucanase Cel12A from Gloeophyllum trabeum Show Its Monomeric Structure and Reveal the Influence of Temperature on the Structural Stability of the Enzyme | en |
dc.type | Artigo | |
dcterms.rightsHolder | Mdpi Ag | |
unesp.author.lattes | 8213371495151651 | |
unesp.author.orcid | 0000-0001-9496-4174[4] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatu | pt |
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