Downhill running excessive training inhibits hypertrophy in mice skeletal muscles with different fiber type composition

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dc.contributor.authorRocha, Alisson L. da
dc.contributor.authorPereira, Bruno C.
dc.contributor.authorPauli, José R.
dc.contributor.authorde Souza, Claudio T.
dc.contributor.authorTeixeira, Giovana R. [UNESP]
dc.contributor.authorLira, Fábio S. [UNESP]
dc.contributor.authorCintra, Dennys E.
dc.contributor.authorRopelle, Eduardo R.
dc.contributor.authorJúnior, Carlos R B
dc.contributor.authorda Silva, Adelino S R
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversity of Far Southern Santa Catarina
dc.date.accessioned2015-12-07T15:38:14Z
dc.date.available2015-12-07T15:38:14Z
dc.date.issued2015-09-18
dc.description.abstractThe aim of this study was to verify the effects of running overtraining protocols performed in downhill, uphill, and without inclination on the proteins related to hypertrophy signaling pathway in extensor digitorum longus (EDL) and soleus of C57BL/6 mice. We also performed histological and stereological analyses. Rodents were divided into control (CT; sedentary mice), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up), and overtrained by running without inclination (OTR). The incremental load, exhaustive, and grip force tests were used as performance evaluation parameters. 36 h after the grip force test, EDL and soleus were removed and immediately used for immunoblotting analysis or stored at -80°C for histological and stereological analyses. For EDL, OTR/down decreased the protein kinase B (Akt) and tuberous sclerosis protein 2 (TSC2) phosphorylation (p), and increased myostatin, receptor-activated Smads (pSMAD2-3), and insulin receptor substrate-1 (pIRS-1; Ser307/636). OTR/down also presented low and high relative proportions of cytoplasm and connective tissue, respectively. OTR/up increased the mammalian target of rapamycin (pmTOR), 70-kDa ribosomal protein S6 kinase 1 (pS6K1) and pSMAD2-3, and decreased pTSC2. OTR decreased pTSC2 and increased pIRS-1 (Ser636). For soleus, OTR/down increased S6 ribosomal protein (pS6RP) and pSMAD2-3, and decreased pIRS-1 (Ser639). OTR/up decreased pS6K1, pS6RP and pIRS-1 (Ser639), and increased pTSC2 (Ser939), and pSMAD2-3. OTR increased pS6RP, 4E-binding protein-1 (p4E-BP1), pTSC2 (Ser939), and pSMAD2-3, and decreased pIRS-1 (Ser639). In summary, OTR/down inhibited the skeletal muscle hypertrophy with concomitant signs of atrophy in EDL. The effects of OTR/up and OTR depended on the analyzed skeletal muscle type. J. Cell. Physiol. 9999: 1-12, 2015. © 2015 Wiley Periodicals, Inc.en
dc.description.affiliationPostgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
dc.description.affiliationSport Sciences Course, Faculty of Applied Sciences, State University of Campinas (UNICAMP), Limeira, São Paulo, Brazil.
dc.description.affiliationExercise Biochemistry and Physiology Laboratory Postgraduate Program in Health Sciences, Health Sciences Unit, University of Far Southern Santa Catarina, Criciúma, Santa Catarina, Brazil.
dc.description.affiliationDepartment of Physical Education, State University of São Paulo (UNESP), Presidente Prudente, São Paulo, Brazil.
dc.description.affiliationSchool of Physical Education and Sport of Ribeirão Preto, USP, Ribeirão Preto, São Paulo, Brazil.
dc.description.affiliationUnespDepartment of Physical Education, State University of São Paulo (UNESP), Presidente Prudente, São Paulo, Brazil.
dc.identifierhttp://dx.doi.org/10.1002/jcp.25197
dc.identifier.citationJournal Of Cellular Physiology, 2015.
dc.identifier.doi10.1002/jcp.25197
dc.identifier.issn1097-4652
dc.identifier.pubmed26381504
dc.identifier.urihttp://hdl.handle.net/11449/131592
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Cellular Physiology
dc.relation.ispartofsjr1,641
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.titleDownhill running excessive training inhibits hypertrophy in mice skeletal muscles with different fiber type compositionen
dc.typeArtigo
dcterms.rightsHolderWiley-Blackwell
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

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