Biochemical and functional properties of a thrombin-like enzyme isolated from Bothrops pauloensis snake venom

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dc.contributor.authorCosta, Fabio L. S.
dc.contributor.authorRodrigues, Renata S.
dc.contributor.authorIzidoro, Luiz F. M.
dc.contributor.authorMenaldo, Danilo L.
dc.contributor.authorHamaguchi, Amelia
dc.contributor.authorHomsi-Brandeburgo, Maria I.
dc.contributor.authorFuly, Andre L.
dc.contributor.authorSoares, Sandro G.
dc.contributor.authorSelistre-de-Araujo, Heloisa S.
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorSoares, Andreimar M.
dc.contributor.authorRodrigues, Veridiana M.
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:12:52Z
dc.date.available2014-05-20T13:12:52Z
dc.date.issued2009-11-01
dc.description.abstractIn the present study, a thrombin-like enzyme named BpSP-I was isolated from Bothrops pauloensis snake venom and its biochemical, enzymatic and pharmacological characteristics were determined. BpSP-I is a glycoprotein that contains both N-linked carbohydrates and sialic acid in its structure, with M(r) = 34,000 under reducing conditions and pI similar to 6.4. The N-terminal sequence of the enzyme (VIGGDECDINEHPFL) showed high similarity with other thrombin-like enzymes from snake venoms. BpSP-I showed high clotting activity upon bovine and human plasma and was inhibited by PMSF, benzamidine and leupeptin. Moreover, this enzyme showed stability when examined at different temperatures (-70 to 37 degrees C), pH values (3-9) or in the presence of divalent metal ions (Ca(2+), Mg(2+), Zn(2+) and Mn(2+)). BpSP-I showed high catalytic activity upon substrates, such as fibrinogen, TAME, S-2238 and S-2288. It also showed kallikrein-like activity, but was unable to act upon factor Xa and plasmin substrates. Indeed, the enzyme did not induce hemorrhage, myotoxicity or edema. Taken together, our data showed that BpSP-I is in fact a thrombin-like enzyme isoform isolated from Bothrops pauloensis snake venom. (C) 2009 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniversidade Federal de Uberlândia (UFU), Inst Genet & Bioquim, BR-38400 Uberlandia, MG, Brazil
dc.description.affiliationUniv São Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Fluminense, Inst Biol, Dept Biol Celular & Mol, Niteroi, RJ, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniversidade Federal de São Carlos (UFSCar), Dept Ciencias Fisiol, BR-13560 São Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, CEVAP, Ctr Estudos Venenos & Anim Peconhentos, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, CEVAP, Ctr Estudos Venenos & Anim Peconhentos, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent725-735
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2009.05.040
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 54, n. 6, p. 725-735, 2009.
dc.identifier.doi10.1016/j.toxicon.2009.05.040
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/11449/783
dc.identifier.wosWOS:000270626900003
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofToxicon
dc.relation.ispartofjcr2.352
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBothrops (neuwiedi) pauloensisen
dc.subjectBlood coagulationen
dc.subjectProteolytic enzymesen
dc.subjectSerine proteinaseen
dc.subjectThrombin-like enzymesen
dc.titleBiochemical and functional properties of a thrombin-like enzyme isolated from Bothrops pauloensis snake venomen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes6840524602748457[10]
unesp.author.orcid0000-0002-9855-5594[10]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos