Molecular detection of fungi of public health importance in wild animals from Southern Brazil

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dc.contributor.authorLosnak, Debora O. [UNESP]
dc.contributor.authorRocha, Francielle R.
dc.contributor.authorAlmeida, Barbara S.
dc.contributor.authorBatista, Keila Z. S.
dc.contributor.authorAlthoff, Sérgio L.
dc.contributor.authorHaupt, Josiane
dc.contributor.authorRuiz, Luciana S.
dc.contributor.authorAnversa, Laís
dc.contributor.authorLucheis, Simone B.
dc.contributor.authorPaiz, Laís M.
dc.contributor.authorDonalisio, Maria Rita
dc.contributor.authorRichini Pereira, Virginia B.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCenter of Biomedical Science
dc.contributor.institutionRegional University of Blumenau
dc.contributor.institutionAutonomous Biologist
dc.contributor.institutionPaulista Agency of Agribusiness Technology
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2018-12-11T17:19:11Z
dc.date.available2018-12-11T17:19:11Z
dc.date.issued2018-07-01
dc.description.abstractSome animals have an important relationship with fungal infections, and searching for pathogens in animal samples may be an opportunity for eco-epidemiological research. Since studies involving wildlife are generally restricted, using samples from road kills is an alternative. The aim of this study was to verify whether pathogenic fungi of public health importance occur in wildlife road kills from Santa Catarina State, Brazil. Organ samples (n = 1063) from 297 animals were analysed according to Polymerase Chain Reaction (PCR) using universal primers to detect fungi in general and, subsequently, using primers specific to Paracoccidioides brasiliensis, Histoplasma capsulatum and Cryptococcus spp. There were 102 samples positive for fungal species. Eight samples were positive for P. brasiliensis, three samples were positive for Cryptococcus spp. and one sample had coinfection by these two fungi. No sample was positive for Histoplasma spp. according to the molecular detection. Genetic sequencing allowed the identification of Fungal sp. in 89 samples, Cryptococcus neoformans in two samples and Aspergillus penicillioides in three samples. This study shows the importance of wild animals in the epidemiology of fungal infections and assists in the mapping of pathogen occurrence in a region that was not previously evaluated.en
dc.description.affiliationDepartment of Tropical Diseases and Imaging Diagnosis Botucatu Medical School Unesp - Univ Estadual Paulista
dc.description.affiliationAdolfo Lutz Institute Center of Regional Laboratories II - Bauru Center of Biomedical Science
dc.description.affiliationLaboratory of Immunology Department of Natural Sciences Regional University of Blumenau
dc.description.affiliationLaboratory of Animal Biology Department of Natural Sciences Regional University of Blumenau
dc.description.affiliationAutonomous Biologist
dc.description.affiliationPaulista Agency of Agribusiness Technology
dc.description.affiliationDepartment of Public Health School of Medical Sciences State University of Campinas—UNICAMP
dc.description.affiliationUnespDepartment of Tropical Diseases and Imaging Diagnosis Botucatu Medical School Unesp - Univ Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/17519-4
dc.format.extent455-463
dc.identifierhttp://dx.doi.org/10.1111/myc.12767
dc.identifier.citationMycoses, v. 61, n. 7, p. 455-463, 2018.
dc.identifier.doi10.1111/myc.12767
dc.identifier.issn1439-0507
dc.identifier.issn0933-7407
dc.identifier.scopus2-s2.0-85044933074
dc.identifier.urihttp://hdl.handle.net/11449/176132
dc.language.isoeng
dc.relation.ispartofMycoses
dc.relation.ispartofsjr1,069
dc.relation.ispartofsjr1,069
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAspergillus penicillioides
dc.subjectCryptococcus spp.
dc.subjectHistoplasma capsulatum
dc.subjectParacoccidioides brasiliensis
dc.subjectpathogenic fungi
dc.subjectPCR
dc.titleMolecular detection of fungi of public health importance in wild animals from Southern Brazilen
dc.typeArtigo
unesp.author.orcid0000-0001-7374-8640[7]
unesp.author.orcid0000-0002-2542-5596[8]
unesp.author.orcid0000-0001-8312-7397[12]

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Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB