In vitro production of bovine embryos using Sigma antioxidant supplement®, α-tocopherol and L-ascorbic acid

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dc.contributor.authorSudano, Mateus José [UNESP]
dc.contributor.authorMattos, Maria Clara Costa
dc.contributor.authorFernandes, C. B.
dc.contributor.authorMazieiro, Rosiaria Rosaria
dc.contributor.authorAlvarenga, Fernanda da Cruz Landim e [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-04-01T18:44:18Z
dc.date.available2016-04-01T18:44:18Z
dc.date.issued2010
dc.description.abstractThis study tested the effect of Sigma antioxidant supplement®, α-tocopherol (vitamin E) and L-ascorbic acid (vitamin C) in the culture medium of bovine embryos. In experiment 1, in vitro produced bovine zygotes were cultured in Human Tubal Fluid (HTF): Eagle’s Basic Medium (BME) with: Group 1 – 50 µm vitamin C; Group 2 – 200 µm vitamin E; Group 3 – 25 µm vitamin C and 100 µm vitamin E; Group 4 – 1 µl/ml Sigma antioxidant supplement®; and the Control group – HTF:BME only. In experiment 2, embryos were cultured in high or low oxygen tension with HTF:BME + Sigma antioxidant supplement® or in HTF:BME alone (Control). The data were analyzed using ANOVA followed by Tukey’s test. The results of experiment 1 showed a negative effect (P < 0.05) of vitamin E on blastocyst production in Group 2 (19.7 ± 0.1%). This effect was reduced in Group 3 by the addition of vitamin C (26.1 ± 0.2%). The use of vitamin C alone (34.9 ± 0.3%) or the Sigma antioxidant supplement® (33.3 ± 0.7%) did not increase (P > 0.05) the number of blastocysts produced compared with the control group (30.1 ± 0.5%). During experiment 2, there was no effect (P > 0.05) from the culture medium or the O2 concentrations used, indicating that the reduction of the O2 concentration did not improve blastocyst production.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Rubião Junior sem número, Rubião Junior, CEP 18618-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, Botucatu, Rubião Junior sem número, Rubião Junior, CEP 18618-000, SP, Brasil
dc.format.extent42-48
dc.identifierhttp://www.cbra.org.br/portal/publicacoes/ar/2010/arjm2010.html
dc.identifier.citationAnimal Reproduction, v. 7, n. 1, p. 42-48, 2010.
dc.identifier.fileISSN1806-9614-2010-07-01-42-48.pdf
dc.identifier.issn1806-9614
dc.identifier.urihttp://hdl.handle.net/11449/137127
dc.language.isoeng
dc.relation.ispartofAnimal Reproduction
dc.relation.ispartofjcr0.991
dc.relation.ispartofsjr0,308
dc.rights.accessRightsAcesso aberto
dc.sourceCurrículo Lattes
dc.subjectIn vitro developmenten
dc.subjectEmbryosen
dc.subjectBovineen
dc.subjectTocopherolen
dc.subjectAscorbic aciden
dc.titleIn vitro production of bovine embryos using Sigma antioxidant supplement®, α-tocopherol and L-ascorbic aciden
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterináriapt

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