Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species

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dc.contributor.authorFalqueto, Silvia Altoe
dc.contributor.authorPitaluga, Bruno Faria
dc.contributor.authorSousa, Janaina Rosa de
dc.contributor.authorTarganski, Sabrina Ketrin
dc.contributor.authorCampos, Mateus Gandra
dc.contributor.authorOliveira Mendes, Tiago Antonio de
dc.contributor.authorSilva, Gilvan Ferreira da
dc.contributor.authorSiqueira Silva, Dulce Helena [UNESP]
dc.contributor.authorSoares, Marcos Antonio
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T12:38:21Z
dc.date.available2021-06-25T12:38:21Z
dc.date.issued2021-02-01
dc.description.abstractThe growing spread of dengue, chikungunya and Zika viruses demand the development of new and environmentally safe control methods for their vector, the mosquito Aedes aegypti. This study aims to find novel larvicidal agents from mutualistic (endophytic and rhizospheric) or edaphic bacteria that have no action against nontarget organisms. Eleven out of the 254 bacterial strains tested were able to kill Ae. aegypti larvae. Larvicidal activity did not depend on presence of cells, since culture supernatants or crude lipopeptide extracts (CLEs) killed the larvae. Bacillus safensis BacI67 and Bacillus paranthracis C21 supernatants were the best performing supernatants, displaying the lowest lethal concentrations (LC50 = 31.11 mu L/mL and 45.84 mu L/mL, respectively). Bacillus velezensis B64a and Bacillus velezensis B15 produced the best performing CLEs (LC50 = 0.11 mg/mL and 0.12 mg/mL, respectively). Mass spectrometry analysis of CLEs detected a mixture of surfactins, iturins, and fengycins. The samples tested were weakly- or non-toxic to mammalian cells (RAW 264.7 macrophages and VERO cells) and non-target organisms (Caenorhabditis elegans, Galleria mellonella, Scenedesmus obliques, and Tetrahymena pyriformis) - especially B. velezensis B15 CLE. The biosynthetic gene clusters related to secondary metabolism identified by whole genome sequencing of the four best performing bacteria strains revealed clusters for bacteriocin, beta-lactone, lanthipeptide, non-ribosomal peptide synthetases, polyketide synthases (PKS), siderophores, T3PKS, type 1 PKS-like, terpenes, thiopeptides, and trans-AT-PKS. Purification of lipopeptides may clarify the mechanisms by which these extracts kill Ae. aegypti larvae.en
dc.description.affiliationUniv Fed Mato Grosso, Dept Bot & Ecol, Av Fernando Correa da Costa 2367, BR-78060900 Cuiaba, Brazil
dc.description.affiliationUniv Fed Vicosa, Dept Bioquim & Biol Mol, Vicosa, MG, Brazil
dc.description.affiliationEmbrapa Amazonia Ocident, Manaus, Amazonas, Brazil
dc.description.affiliationUniv Estadual Paulista, Ctr Inovacao Biodiversidade & Farmacos, Inst Quim, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Ctr Inovacao Biodiversidade & Farmacos, Inst Quim, Araraquara, SP, Brazil
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT, Mato Grosso, MT, Brazil)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT, Mato Grosso, MT, Brazil): 0415241/2016
dc.description.sponsorshipIdCNPq: 307288/2018-8
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1016/j.jip.2020.107525
dc.identifier.citationJournal Of Invertebrate Pathology. San Diego: Academic Press Inc Elsevier Science, v. 179, 10 p., 2021.
dc.identifier.doi10.1016/j.jip.2020.107525
dc.identifier.issn0022-2011
dc.identifier.urihttp://hdl.handle.net/11449/210057
dc.identifier.wosWOS:000620238500001
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Invertebrate Pathology
dc.sourceWeb of Science
dc.subjectBacillus safensis
dc.subjectBacillus paranthracis
dc.subjectBacillus velezensis
dc.subjectLipopeptide
dc.subjectGenome mining
dc.titleBacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target speciesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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