Meio de cultura e tipo de explante no estabelecimento in vitro de espécies de maracujazeiro

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Faria, Gláucia Amorim [UNESP]
De Carvalho Costa, Maria Angélica Pereira
Da Silva Ledo, Carlos Alberto
Junghans, Tatiana Góes
Da Silva Souza, Antônio
Da Cunha, Mario Augusto Pinto

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Brazil is one of the main centers of genetic variability dispersion of the Passiflora genera. Its self incompatibility as well as disease incidence in its leaves and root system and, deforestation and monocultivation, promote loss of genetic material. Considering the risk of genetic erosion, the conservation of the variability in germplasm banks, which is of great interest in plant breeding, is necessary. Studies regarding the type of expiant and concentration of the culture media are necessary in order to determine protocols of establishment and in vitro conservation of passion-fruit germplasm. The objective of the present work was to evaluate the influence of the salt and nutrient concentration in the MS culture medium and types of expiants in the establishment and growth of the Passion fruit species: Passiflora giberti N. E.Brown, P. edulis Sims and P. laurifolia L. Each Passiflora species presented its own characteristics regarding in vitro development. The complete MS medium and nodal segments the second axilliary bud promoted better development of the genotypes studied.



Bud position, Micropropagation, Passiflora spp., conservation genetics, disease incidence, experimental study, fruit, genetic variation, genotype, germplasm, nutrient uptake, plant breeding, root system, salt, self incompatibility, vine, Brazil, South America, Passiflora, Passiflora edulis, Passiflora giberti, Passiflora laurifolia, biological characteristic, biological property, biotechnology, disease, erosion control

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Bragantia, v. 66, n. 4, p. 535-543, 2007.