Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms

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dc.contributor.authorKoga-Ito, Cristiane Yumi [UNESP]
dc.contributor.authorLeite, Lady Daiane Pereira
dc.contributor.authorOliveira, Maria Alcionéia Carvalho de
dc.contributor.authorVegian, Mariana Raquel da Cruz [UNESP]
dc.contributor.authorSampaio, Aline da Graça [UNESP]
dc.contributor.authorNishime, Thalita Mayumi Castaldelli [UNESP]
dc.contributor.authorKostov, Konstantin Georgiev
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2022-01-20T17:48:24Z
dc.date.available2022-01-20T17:48:24Z
dc.date.issued2021-09-25
dc.description.abstract: The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.en
dc.description.affiliationScience Applied to Oral Health, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos
dc.description.affiliationDepartment of Environment Engineering, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos
dc.description.affiliationOral Biopathology, Graduate Program of Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos
dc.description.affiliationLeibniz Institute for Plasma Science and Technology
dc.description.affiliationDepartment of Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (Unesp), Guaratinguetá
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2019/05856-7
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 308127/2018-80
dc.identifier.citationLEITE, L. D. P.; OLIVEIRA, M. A. C.; VEGIAN, M. R. C.; SAMPAIO, A. G.; NISHIME, T. M. C.; KOSTOV, K. G.; KOGA-ITO, C. Y. Effect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilms. Molecules, v. 26, n. 19, p. 5815, 2021.pt
dc.identifier.issn1420-3049
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.urihttp://hdl.handle.net/11449/215986
dc.language.isoeng
dc.publisherMDPI
dc.relationhttps://doi.org/10.3390/molecules26195815pt
dc.relation.ispartofMoleculespt
dc.rights.accessRightsAcesso aberto
dc.subjectCandida albicansen
dc.subjectAntifungalen
dc.subjectCold plasmaen
dc.titleEffect of cold atmospheric plasma jet associated to polyene antifungals on Candida albicans biofilmsen
dc.typeArtigo
unesp.author.lattes6543563161403421[1]
unesp.author.orcid0000-0002-2416-2173[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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Artigos - Engenharia Ambiental - São José dos Campos