Evaluation of different protocols for anatomical studies in Parmeliaceae (lichenized Ascomycota)

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dc.contributor.authorBarbosa, Suzana Bissacot [UNESP]
dc.contributor.authorMarcelli, Marcelo Pinto
dc.contributor.authorMachado, Silvia Rodrigues [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Bot
dc.date.accessioned2014-05-20T13:48:44Z
dc.date.available2014-05-20T13:48:44Z
dc.date.issued2009-02-01
dc.description.abstractAnatomical information on most lichen species, including Parmeliaceae species, is scarce and superficial. This is partially due to the technical difficulties found during the preparation of samples and sections suitable for optical microscopy analysis. There is a lack of pictures of anatomical sections as well as detailed anatomical protocols related to the sample processing made by specialized plant anatomists in literature. This work aimed to look for a standardized histological technique and to develop a protocol for histological studies of foliose Parmeliaceae under light microscopy. Four common pantropical species of Parmeliaceae, abundant in the Brazilian cerrado vegetation, were processed in several ways in relation to fixing and inclusion media, sectioning thicknesses, and staining, including both fresh and 2-year-old herbarium specimens. The best technique found for anatomical studies of Parmeliaceae under light microscopy is represented by 2 or 3 mu m thick sections of fresh or herbarium samples, desiccated and fixed in FAA 50, infiltrated and embedded in glycol-methacrylate resin (Leica), and stained by Toluidine Blue or Methylene Blue. (c) 2008 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Bot, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationInst Bot, Secao Micol & Liquenol, BR-01061970 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Bot, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 00/12469-3
dc.format.extent218-225
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2008.09.001
dc.identifier.citationMicron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 40, n. 2, p. 218-225, 2009.
dc.identifier.doi10.1016/j.micron.2008.09.001
dc.identifier.issn0968-4328
dc.identifier.lattes2653496390637757
dc.identifier.urihttp://hdl.handle.net/11449/17352
dc.identifier.wosWOS:000262072000007
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMicron
dc.relation.ispartofjcr1.728
dc.relation.ispartofsjr0,624
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAnatomyen
dc.subjectLichensen
dc.subjectLight microscopyen
dc.subjectProtocolen
dc.subjectHistologyen
dc.subjectParmeliaceaeen
dc.titleEvaluation of different protocols for anatomical studies in Parmeliaceae (lichenized Ascomycota)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes2653496390637757
unesp.author.orcid0000-0002-0065-1933[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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