Integrating cytogenetics and genomics in comparative evolutionary studies of cichlid fish

Página do item simplificado
dc.contributor.authorMazzuchelli, Juliana [UNESP]
dc.contributor.authorKocher, Thomas David
dc.contributor.authorYang, Fengtang
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Maryland
dc.contributor.institutionWellcome Trust Sanger Inst
dc.date.accessioned2014-05-20T13:52:28Z
dc.date.available2014-05-20T13:52:28Z
dc.date.issued2012-09-09
dc.description.abstractBackground: The availability of a large number of recently sequenced vertebrate genomes opens new avenues to integrate cytogenetics and genomics in comparative and evolutionary studies. Cytogenetic mapping can offer alternative means to identify conserved synteny shared by distinct genomes and also to define genome regions that are still not fine characterized even after wide-ranging nucleotide sequence efforts. An efficient way to perform comparative cytogenetic mapping is based on BAC clones mapping by fluorescence in situ hybridization. In this report, to address the knowledge gap on the genome evolution in cichlid fishes, BAC clones of an Oreochromis niloticus library covering the linkage groups (LG) 1, 3, 5, and 7 were mapped onto the chromosomes of 9 African cichlid species. The cytogenetic mapping data were also integrated with BAC-end sequences information of O. niloticus and comparatively analyzed against the genome of other fish species and vertebrates.Results: The location of BACs from LG1, 3, 5, and 7 revealed a strong chromosomal conservation among the analyzed cichlid species genomes, which evidenced a synteny of the markers of each LG. Comparative in silico analysis also identified large genomic blocks that were conserved in distantly related fish groups and also in other vertebrates.Conclusions: Although it has been suggested that fishes contain plastic genomes with high rates of chromosomal rearrangements and probably low rates of synteny conservation, our results evidence that large syntenic chromosome segments have been maintained conserved during evolution, at least for the considered markers. Additionally, our current cytogenetic mapping efforts integrated with genomic approaches conduct to a new perspective to address important questions involving chromosome evolution in fishes.en
dc.description.affiliationUNESP São Paulo State Univ, Biosci Inst, Dept Morphol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Maryland, Dept Biol, College Pk, MD 20742 USA
dc.description.affiliationWellcome Trust Sanger Inst, Cambridge CB10 1SA, England
dc.description.affiliationUnespUNESP São Paulo State Univ, Biosci Inst, Dept Morphol, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipWellcome Trust
dc.description.sponsorshipIdWellcome Trust: WT098051
dc.format.extent14
dc.identifierhttp://dx.doi.org/10.1186/1471-2164-13-463
dc.identifier.citationBmc Genomics. London: Biomed Central Ltd., v. 13, p. 14, 2012.
dc.identifier.doi10.1186/1471-2164-13-463
dc.identifier.fileWOS000309413900001.pdf
dc.identifier.issn1471-2164
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/18742
dc.identifier.wosWOS:000309413900001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBMC Genomics
dc.relation.ispartofjcr3.730
dc.relation.ispartofsjr2,110
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCichlidaeen
dc.subjectGenome evolutionen
dc.subjectMolecular cytogeneticsen
dc.subjectChromosomeen
dc.subjectLinkage groupsen
dc.subjectBACsen
dc.titleIntegrating cytogenetics and genomics in comparative evolutionary studies of cichlid fishen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes8858800699425352[4]
unesp.author.orcid0000-0003-3534-974X[4]
unesp.author.orcid0000-0002-7547-0133[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000309413900001.pdf
Tamanho:
688.68 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Morfologia - IBB