The homeostasis of iron, copper, and zinc in paracoccidioides brasiliensis, cryptococcus neoformans var. Grubii, and cryptococcus gattii: a comparative analysis

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dc.contributor.authorSilva, Merelle Garcia
dc.contributor.authorSchrank, Augusto
dc.contributor.authorBailão, Elisa Flávia L. C.
dc.contributor.authorBailão, Alexandre Melo
dc.contributor.authorBorges, Clayton Luiz
dc.contributor.authorStaats, Charley Christian
dc.contributor.authorParente, Juliana Alves
dc.contributor.authorPereira, Maristela
dc.contributor.authorIzaac, Silvia Maria Salem
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.authorOliveira, Roseli Maria Zancopé
dc.contributor.authorSilva, Livia K. Rosa e
dc.contributor.authorNosanchuk, Joshua D.
dc.contributor.authorVainstein, Marilene Henning
dc.contributor.authorSoares, Célia Maria de Almeida
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS )
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundação Oswaldo Cruz (Fiocruz)
dc.contributor.institutionAlbert Einstein College of Medicine
dc.date.accessioned2016-01-28T16:56:17Z
dc.date.available2016-01-28T16:56:17Z
dc.date.issued2011
dc.description.abstractIron, copper, and zinc are essential for all living organisms. Moreover, the homeostasis of these metals is vital to microorganisms during pathogenic interactions with a host. Most pathogens have developed specific mechanisms for the uptake of micronutrients from their hosts in order to counteract the low availability of essential ions in infected tissues. We report here an analysis of genes potentially involved in iron, copper, and zinc uptake and homeostasis in the fungal pathogens Paracoccidioides brasiliensis, Cryptococcus neoformans var. grubii, and Cryptococcus gattii. Although prior studies have identified certain aspects of metal regulation in Cryptococcus species, little is known regarding the regulation of these elements in P. brasiliensis. We also present amino acid sequences analyses of deduced proteins in order to examine possible conserved domains. The genomic data reveals, for the first time, genes associated to iron, copper, and zinc assimilation and homeostasis in P. brasiliensis. Furthermore, analyses of the three fungal species identified homologs to genes associated with high-affinity uptake systems, vacuolar and mitochondrial iron storage, copper uptake and reduction, and zinc assimilation. However, homologs to genes involved in siderophore production were only found in P. brasiliensis. Interestingly, in silico analysis of the genomes of P. brasiliensis Pb01, Pb03, and Pb18 revealed significant differences in the presence and/or number of genes involved in metal homeostasis, such as in genes related to iron reduction and oxidation. The broad analyses of the genomes of P. brasiliensis, C. neoformans var. grubii, and C. gattii for genes involved in metal homeostasis provide important groundwork for numerous interesting future areas of investigation that are required in order to validate and explore the function of the identified genes and gene pathways.en
dc.description.affiliationUniversidade Federal de Goiás, Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Goiânia, Goiás, Brazil
dc.description.affiliationUniversidade Federal do Rio Grande do Sul, Laboratório de Biologia Molecular, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul, Brazil
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, R. Expedicionários do Brasil, 1621 - Laboratório de Micologia Clínica, Centro, CEP 14801902, SP, Brasil
dc.description.affiliationFundação Oswaldo Cruz, Laboratório de Micologia, Instituto de Pesquisa Evandro Chagas, Rio De Janeiro, Brazil
dc.description.affiliationAlbert Einstein College of Medicine, Division of Infectious Diseases, Department of Medicine, Bronx, NY, USA
dc.description.affiliationAlbert Einstein College of Medicine, Department Microbiology and Immunology, Bronx, NY, USA
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, R. Expedicionários do Brasil, 1621 - Laboratório de Micologia Clínica, Centro, CEP 14801902, SP, Brasil
dc.description.sponsorshipMCT/FINEP/Rede GENOPROT
dc.description.sponsorshipIdMCT/FINEP/Rede GENOPROT: 01.07.0552.00
dc.format.extent1-19
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2011.00049
dc.identifier.citationFrontiers in Microbiology, v. 2, n. 49, p. 1-19, 2011.
dc.identifier.doi10.3389/fmicb.2011.00049
dc.identifier.issn1664-302X
dc.identifier.lattes0134821761458628
dc.identifier.lattes6238654594421708
dc.identifier.lattes1152828531273019
dc.identifier.lattes8867708267053410
dc.identifier.lattes8972540207861201
dc.identifier.lattes8539946335852637
dc.identifier.lattes1345781867765758
dc.identifier.lattes6034625575894077
dc.identifier.lattes5415221996976886
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.urihttp://hdl.handle.net/11449/133713
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiology
dc.relation.ispartofjcr4.019
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.titleThe homeostasis of iron, copper, and zinc in paracoccidioides brasiliensis, cryptococcus neoformans var. Grubii, and cryptococcus gattii: a comparative analysisen
dc.typeArtigo
unesp.author.lattes0134821761458628
unesp.author.lattes6238654594421708
unesp.author.lattes1152828531273019
unesp.author.lattes8867708267053410
unesp.author.lattes8972540207861201
unesp.author.lattes8539946335852637
unesp.author.lattes1345781867765758
unesp.author.lattes6034625575894077
unesp.author.lattes5415221996976886
unesp.author.orcid0000-0002-8059-0826[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicaspt

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