Effect of the pH on the Antibacterial Potential and Cytotoxicity of Different Plasma-Activated Liquids

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dc.contributor.authorSampaio, Aline da Graça [UNESP]
dc.contributor.authorChiappim, William [UNESP]
dc.contributor.authorMilhan, Noala Vicensoto Moreira [UNESP]
dc.contributor.authorBotan Neto, Benedito
dc.contributor.authorPessoa, Rodrigo
dc.contributor.authorKoga-Ito, Cristiane Yumi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTechnological Institute of Aeronautics
dc.date.accessioned2023-07-29T15:15:29Z
dc.date.available2023-07-29T15:15:29Z
dc.date.issued2022-11-01
dc.description.abstractIn this study, different plasma-activated liquids were evaluated for their antimicrobial effects against Escherichia coli, as well as for their cytotoxicity on mammalian cells. The PALs were prepared from distilled (DIS), deionized (DI), filtered (FIL), and tap (TAP) water. Additionally, 0.9% NaCl saline solution (SAL) was plasma-activated. These PALs were prepared using 5 L/min air gliding arc plasma jet for up to 60.0 min of exposure. Subsequently, the physicochemical properties, such as, the oxidation-reduction potential (ORP), the pH, the conductivity, and the total dissolved solids (TDS) were characterized by a water multiparameter. The PALs obtained showed a drastic decrease in the pH with increasing plasma exposure time, in contrast, the conductivity and TDS increased. In a general trend, the UV-vis analyses identified a higher production of the following reactive species of nitrogen and oxygen (RONS), HNO2, H2O2, NO3−, and NO2−. Except for the plasma-activated filtered water (PAW-FIL), where there was a change in the position of NO2− and NO3− at some pHs, The higher production of HNO2 and H2O2-reactive species was observed at a low pH. Finally, the standardized suspensions of Escherichia coli were exposed to PAL for up to 60.0 min. The plasma-activated deionized water (PAW-DI pH 2.5), plasma-activated distilled water (PAW-DIS pH 2.5 and 3), and plasma-activated tap water (PAW-TAP 3.5) showed the best antimicrobial effects at exposure times of 3.0, 10.0, and 30.0 min, respectively. The MTT analysis demonstrated low toxicity of all of the PAL samples. Our results indicate that the plasma activation of different liquids using the gliding arc system can generate specific physicochemical conditions that produce excellent antibacterial effects for E. coli with a safe application, thus bringing future contributions to creating new antimicrobial protocols.en
dc.description.affiliationScience Applied to Oral Health Graduate Program of Institute of Science and Technology UNESP
dc.description.affiliationLaboratory of Plasmas and Applications Department of Physics Faculty of Engineering and Sciences São Paulo State University (UNESP)
dc.description.affiliationPlasmas and Processes Laboratory Department of Physics Technological Institute of Aeronautics, Praça Marechal Eduardo Gomes 50
dc.description.affiliationDepartment of Environment Engineering Institute of Science and Technology São Paulo State University (UNESP)
dc.description.affiliationUnespScience Applied to Oral Health Graduate Program of Institute of Science and Technology UNESP
dc.description.affiliationUnespLaboratory of Plasmas and Applications Department of Physics Faculty of Engineering and Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Environment Engineering Institute of Science and Technology São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 309762/2021-9
dc.identifierhttp://dx.doi.org/10.3390/ijms232213893
dc.identifier.citationInternational Journal of Molecular Sciences, v. 23, n. 22, 2022.
dc.identifier.doi10.3390/ijms232213893
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85142841207
dc.identifier.urihttp://hdl.handle.net/11449/249415
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectantimicrobial effect
dc.subjectdeionized water
dc.subjectdistilled water
dc.subjectEscherichia coli
dc.subjectfiltered water
dc.subjectgliding arc discharge
dc.subjectplasma-activated liquid (PAL)
dc.subjectplasma-activated water (PAW)
dc.subjectsaline
dc.subjecttoxicity
dc.titleEffect of the pH on the Antibacterial Potential and Cytotoxicity of Different Plasma-Activated Liquidsen
dc.typeArtigo
unesp.author.orcid0000-0001-9453-3905[1]
unesp.author.orcid0000-0002-9137-8476[3]
unesp.author.orcid0000-0001-7600-9747[5]
unesp.author.orcid0000-0002-2416-2173[6]

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