Characterization of bovine transcripts preferentially expressed in testis and with a putative role in spermatogenesis

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dc.contributor.authorAlves, B. C. A.
dc.contributor.authorTobo, P. R.
dc.contributor.authorRodrigues, R.
dc.contributor.authorRuiz, J. C.
dc.contributor.authorHossepian de Lima, V. F. M. [UNESP]
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFiocruz MS
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:29Z
dc.date.available2014-05-20T13:15:29Z
dc.date.issued2011-10-01
dc.description.abstractAlthough the number of genes known to be associated with bovine spermatogenesis has increased in the past few years, regulation of this biological process remains poorly understood. Therefore, discovery of new male fertility genetic markers is of great value for assisted selection in commercially important cattle breeds, e.g., Nelore, that have delayed reproductive maturation and low fertility rates. The objective of the present study was to identify sequences associated with spermatogenesis that could be used as fertility markers. With RT-PCR, the following five transcripts preferentially expressed in adult testis were detected: TET(656) detected only in adult testis; TET(868) and TET(515) expressed preferentially in adult testis but also detected in fetal gonads of both sexes; and TET(456) and TET(262). expressed primarily in the testis, but also present in very low amounts in somatic tissues. Based on their homologies and expression profiles, we inferred that they had putative roles in spermatogenesis. Detection of sequences differentially expressed in testis, ovary, or both, was a useful approach for identifying new genes related to bovine spermatogenesis. The data reported here contributed to discovery of gene pathways involved in bovine spermatogenesis, with potential for prediction of fertility. (C) 2011 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Med, Dept Pediat, BR-05403900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Ctr Pesquisas Biotecnol, BR-05508900 São Paulo, Brazil
dc.description.affiliationFiocruz MS, CPqRR, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, Brazil
dc.description.affiliationUNESP, Fac Ciencias Agr & Vet, Dept Anim Reprod, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agr & Vet, Dept Anim Reprod, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 04/14738-2
dc.description.sponsorshipIdCNPq: 305635/2009-3
dc.description.sponsorshipIdCNPq: 300996/2006-7
dc.format.extent991-998
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2011.04.027
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 76, n. 6, p. 991-998, 2011.
dc.identifier.doi10.1016/j.theriogenology.2011.04.027
dc.identifier.fileWOS000294833800002.pdf
dc.identifier.issn0093-691X
dc.identifier.lattes9313529255111902
dc.identifier.urihttp://hdl.handle.net/11449/2613
dc.identifier.wosWOS:000294833800002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectSpermatogenesisen
dc.subjectTestis gene expressionen
dc.subjectFertility markeren
dc.subjectDifferential gene expressionen
dc.subjectqPCRen
dc.titleCharacterization of bovine transcripts preferentially expressed in testis and with a putative role in spermatogenesisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes9313529255111902[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV