Genetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markers

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dc.contributor.authorSantos, Mirella F. C.
dc.contributor.authorRibolla, Paulo E. M. [UNESP]
dc.contributor.authorAlonso, Diego P. [UNESP]
dc.contributor.authorAndrade-Filho, José D.
dc.contributor.authorCasaril, Aline E.
dc.contributor.authorFerreira, Alda M. T.
dc.contributor.authorFernandes, Carlos E. S.
dc.contributor.authorBrazil, Reginaldo P.
dc.contributor.authorOliveira, Alessandra G.
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCCPQRR-FIOCRUZ
dc.contributor.institutionFIOCRUZ
dc.date.accessioned2014-05-27T11:30:41Z
dc.date.available2014-05-27T11:30:41Z
dc.date.issued2013-09-16
dc.description.abstractBackground:Lutzomyia longipalpis (Diptera: Psychodidae) is the major vector of Leishmania (Leishmania) infantum and thus plays a crucial role in the epidemiology of American visceral leishmaniasis (AVL). This vector is the best studied species of sand fly in the Neotropical region. Many studies claim that this vector is in fact a species complex; however there is still no consensus regarding the number of species that belong into this complex or the geographical distribution of sibling species. The aim of the present study was to analyze the genetic relationships within Lu. longipalpis populations in the state of Mato Grosso do Sul (MS), Brazil.Methodology/Principal Findings:We collected 30 Lu. longipalpis (15 females and 15 males) from five localities (Campo Grande, Três Lagoas, Aquidauana, Miranda and Bonito) and 30 Lu. Cruzi from Corumbá, totaling 180 sandflies from MS, and 30 Lu. longipalpis from Estrela de Alagoas, state of Alagoas (AL), Northeast Brazil. We show that eight previously described microsatellite loci were sufficient in distinguishing Lu. longipalpis from Lu. Cruzi, which is a closely related species, and in differentiating between Lu. longipalpis collected in MS versus Estrela de Alagoas. Analyses of the genotypes revealed introgression between sympatric Lu. longipalpis and Lu. Cruzi.Conclusions/Significance:Our findings support the hypothesis of cryptic species within the Lu. longipalpis complex. Furthermore, our data revealed introgression between Lu. longipalpis and Lu. cruzi. This phenomenon should be further investigated to determine the level and incidence of hybridization between these two species. We also demonstrated that microsatellite markers are a powerful tool for differentiating sand fly populations and species. The present study has elucidated the population structure of Lu. longipalpis in MS and, by extension, the Neotropical Lu. longipalpis complex itself. © 2013 Santos et al.en
dc.description.affiliationPrograma de Pós-Graduação em Doenças Infecciosas e Parasitárias Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do Sul
dc.description.affiliationDepartamento de Parasitologia Universidade Estadual Paulista Júlio de Mesquita Filho Unesp, Botucatu, São Paulo
dc.description.affiliationReferencia Nacional e Internacional de Flebotomíneos Centro de Pesquisas René Rachou CCPQRR-FIOCRUZ, Belo Horizonte, Minas Gerais
dc.description.affiliationLaboratório de Imunologia Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do Sul
dc.description.affiliationLaboratório de Patologia Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do Sul
dc.description.affiliationLaboratório de Doenças Parasitárias Instituto Oswaldo Cruz FIOCRUZ, Rio de Janeiro, Rio de Janeiro
dc.description.affiliationLaboratório de Parasitologia Universidade Federal de Mato Grosso do Sul UFMS, Campo Grande, Mato Grosso do Sul
dc.description.affiliationUnespDepartamento de Parasitologia Universidade Estadual Paulista Júlio de Mesquita Filho Unesp, Botucatu, São Paulo
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0074268
dc.identifier.citationPLoS ONE, v. 8, n. 9, 2013.
dc.identifier.doi10.1371/journal.pone.0074268
dc.identifier.file2-s2.0-84884185511.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes3577149748456880
dc.identifier.orcid0000-0001-8735-6090
dc.identifier.scopus2-s2.0-84884185511
dc.identifier.urihttp://hdl.handle.net/11449/76566
dc.identifier.wosWOS:000324494000111
dc.language.isoeng
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleGenetic Structure of Lutzomyia longipalpis Populations in Mato Grosso Do Sul, Brazil, Based on Microsatellite Markersen
dc.typeArtigo
dcterms.licensehttp://www.plos.org/open-access/
unesp.author.lattes3577149748456880[2]
unesp.author.orcid0000-0001-8735-6090[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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