Histological analysis and gene expression profile following augmentation of the anterior maxilla using rhBMP-2/ACS versus autogenous bone graft

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dc.contributor.authorde Freitas, Rubens Moreno
dc.contributor.authorSusin, Cristiano
dc.contributor.authorTamashiro, Wirla Maria da Silva Cunha
dc.contributor.authorChaves de Souza, João Antonio
dc.contributor.authorMarcantonio, Claudio [UNESP]
dc.contributor.authorWikesjö, Ulf ME
dc.contributor.authorPereira, Luís Antônio Violin Dias
dc.contributor.authorMarcantonio, Elcio [UNESP]
dc.contributor.institutionILAPEO - Latin American Institute of Dental Research and Education
dc.contributor.institutionAugusta University
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionDental School
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:06:20Z
dc.date.available2018-12-11T17:06:20Z
dc.date.issued2016-12-01
dc.description.abstractAim: The objective of this report was to present histological characteristics and gene expression profile of newly formed bone following horizontal augmentation of the atrophic anterior maxilla using recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge carrier (rhBMP-2/ACS) versus an autogenous bone graft (ABG). Methods: Bone core biopsies from 24 subjects participating in a randomized clinical trial were obtained at dental implant placement, 6 months following alveolar ridge augmentation using rhBMP-2/ACS (rhBMP-2 at 1.5 mg/ml; total dose 4.2 mg) or a particulate ABG harvested from the mandibular retro-molar region. A titanium mesh was used to provide wound stability and space for bone formation. Analysis included histological/histometric observations and gene expression profile of the newly formed bone. Results: rhBMP-2/ACS yielded bone marrow rich in capillaries, undifferentiated cells and bone lining cells compared with the ABG (p = 0.002). Whereas no significant differences were observed in total bone fraction (p = 0.53), non-vital bone particles trapped in lamellar vital bone were observed in the ABG group (p < 0.001). Real-time PCR showed greater BMP-2 and RUNX2 expression for rhBMP-2/ACS over the ABG (p = 0.001 and 0.0021, respectively), while the ABG exhibited greater expression of RANKL:OPG, BSP and OPN over rhBMP-2/ACS (p = 0.01, 0.005 and 0.0009, respectively). Conclusions: Our observations suggest that formative biological processes explain bone formation following implantation of rhBMP-2/ACS, whereas remodelling, resorptive/formative processes, characterizes sites receiving ABGs.en
dc.description.affiliationDepartment of Post-Graduation ILAPEO - Latin American Institute of Dental Research and Education
dc.description.affiliationLaboratory for Applied Periodontal & Craniofacial Regeneration (LAPCR) Departments of Periodontics and Oral Biology Dental College of Georgia Augusta University
dc.description.affiliationDepartment of Orthopaedic Surgery Medical College of Georgia Augusta University
dc.description.affiliationDepartment of Genetics and Evolution and Bioagent UNICAMP – State University of Campinas Institute of Biology
dc.description.affiliationDepartment of Stomatological Sciences – Periodontology UFG – Federal University of Goias Dental School
dc.description.affiliationDepartment of Diagnostic and Surgery – Periodontics UNESP - Univ Estadual Paulista Araraquara Dental School
dc.description.affiliationDepartment of Biochemistry and Tissue Biology UNICAMP – State University of Campinas Institute of Biology
dc.description.affiliationUnespDepartment of Diagnostic and Surgery – Periodontics UNESP - Univ Estadual Paulista Araraquara Dental School
dc.format.extent1200-1207
dc.identifierhttp://dx.doi.org/10.1111/jcpe.12601
dc.identifier.citationJournal of Clinical Periodontology, v. 43, n. 12, p. 1200-1207, 2016.
dc.identifier.doi10.1111/jcpe.12601
dc.identifier.issn1600-051X
dc.identifier.issn0303-6979
dc.identifier.scopus2-s2.0-84990229992
dc.identifier.urihttp://hdl.handle.net/11449/173570
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Periodontology
dc.relation.ispartofsjr2,079
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectalveolar ridge augmentation
dc.subjectautogenous bone graft
dc.subjectrandomized controlled trial
dc.subjectrecombinant human bone morphogenetic protein
dc.subjectrhBMP-2
dc.titleHistological analysis and gene expression profile following augmentation of the anterior maxilla using rhBMP-2/ACS versus autogenous bone graften
dc.typeArtigo
unesp.author.orcid0000-0002-8053-2407[4]
unesp.author.orcid0000-0003-1607-0583[6]

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