Responses of dental pulp cells to a less invasive bleaching technique applied to adhesive restored teeth

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dc.contributor.authorSoares, Diana Gabriela de Souza
dc.contributor.authorSacono, Nancy Tomoko
dc.contributor.authorRibeiro, Ana Paula Dias
dc.contributor.authorBasso, Fernanda Gonçalves
dc.contributor.authorScheffel, Débora Lopes Salles
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:12:13Z
dc.date.available2015-08-06T16:12:13Z
dc.date.issued2015
dc.description.abstractTo assess the cytotoxicity of 35% hydrogen peroxide (HP) bleaching gel applied for 15 min to sound or restored teeth with two-step self-etching adhesive systems and composite resin. Materials and Methods: Sound and restored enamel/dentin disks were stored in water for 24 h or 6 months + thermocycling. The disks were adapted to artificial pulp chambers and placed in compartments containing culture medium. Immediately after bleaching, the culture medium in contact with dentin was applied for 1 h to previously cultured odontoblast-like MDPC-23 cells. Thereafter, cell viability (MTT assay) and morphology (SEM) were assessed. Data were analyzed by two-way ANOVA and Tukey's test (a = 5%). Results: In comparison to the negative control group (no treatment), no significant cell viability reduction occurred in those groups in which sound teeth were bleached. However, a significant decrease in cell viability was observed in the adhesive-restored bleached groups compared to negative control. No significant difference among bleached groups was observed with respect to the presence of restoration and storage time. Conclusion: The application of 35% HP bleaching gel to sound teeth for 15 min does not cause toxic effects in pulp cells. When this bleaching protocol was performed in adhesive-restored teeth, a significant toxic effect occurred.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.format.extent155-161
dc.identifierhttp://jad.quintessenz.de/index.php?doc=abstract&abstractID=33892/
dc.identifier.citationJournal of Adhesive Dentistry, v. 17, p. 155-161, 2015.
dc.identifier.doi10.3290/j.jad.a33892
dc.identifier.issn1461-5185
dc.identifier.lattes1531215699841687
dc.identifier.lattes8207097271172991
dc.identifier.lattes3147542424193452
dc.identifier.lattes2437404933179101
dc.identifier.lattes4517484241515548
dc.identifier.urihttp://hdl.handle.net/11449/125488
dc.language.isoeng
dc.relation.ispartofJournal of Adhesive Dentistry
dc.relation.ispartofjcr1.691
dc.relation.ispartofsjr0,839
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectLaboratory researchen
dc.subjectToxicityen
dc.subjectOdontoblastsen
dc.subjectTooth bleachingen
dc.subjectAdhesivesen
dc.titleResponses of dental pulp cells to a less invasive bleaching technique applied to adhesive restored teethen
dc.typeArtigo
unesp.author.lattes1531215699841687
unesp.author.lattes8207097271172991
unesp.author.lattes3147542424193452
unesp.author.lattes2437404933179101
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentClínica Infantilpt

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