Characterisation and origins of melanin-concentrating hormone immunoreactive fibres of the posterior lobe of the pituitary and median eminence during lactation in the Long-Evans rat

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dc.contributor.authorCosta, Helder C.
dc.contributor.authorDa-Silva, Joelcimar M.
dc.contributor.authorDiniz, Giovanne B.
dc.contributor.authorMotta-Teixeira, Livia C.
dc.contributor.authorDa-Silva, Renata J.
dc.contributor.authorBattagello, Daniella S.
dc.contributor.authorSita, Luciane V.
dc.contributor.authorde-Moraes Machado, Carla [UNESP]
dc.contributor.authorHorta-Júnior, José A. C. [UNESP]
dc.contributor.authorBittencourt, Jackson C.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:33:55Z
dc.date.available2019-10-06T16:33:55Z
dc.date.issued2019-01-01
dc.description.abstractAlthough the melanin-concentrating hormone (MCH) and its coding mRNA are predominantly found in the tuberal hypothalamus, there is detectable synthesis of MCH in the preoptic hypothalamus exclusively in lactating dams, suggesting a participation of MCH in the alterations that take place after parturition. Also implicated in the dam physiology is oxytocin, a neurohormone released from the posterior pituitary that is necessary for milk ejection. Because the projection fields from oxytocin-immunoreactive (-IR) neurones and the mediobasal preoptic hypothalamus overlap and MCH-IR neurones are found in proximity to oxytocin neurones, we investigated the spatial relationship between MCH and oxytocin fibres. Accordingly, we employed multiple immunohistochemistry labelling for MCH and oxytocin for light and electron microscopy techniques, in addition to i.v. tracer injection combined with in situ hybridisation to identify MCH neurones that project to neurosecretory areas. As described for other strains, lactating Long-Evans dams also display immunoreactivity for MCH in the preoptic hypothalamus on days 12 and 19 of lactation. The appearance of these neurones is contemporaneous with an increase in MCH-IR fibres in both the internal layer of the median eminence and the posterior pituitary. In both regions, MCH- and oxytocin-IR fibres were found in great proximity, although there was no evidence for synaptic interaction between these two populations at the ultrastructural level. The tracer injection revealed that only mediobasal preoptic MCH neurones project to the posterior pituitary, suggesting a neuroendocrine-modulatory role for this population. When taken together, the results obtained in the present study indicate that neuroplasticity events at the mediobasal preoptic hypothalamus that occur during late lactation may be part of a neuroendocrinology control loop involving both MCH and oxytocin.en
dc.description.affiliationLaboratory of Chemical Neuroanatomy Department of Anatomy Institute of Biomedical Sciences University of São Paulo (USP)
dc.description.affiliationDepartment of Physiology and Biophysics Institute of Biomedical Sciences University of São Paulo
dc.description.affiliationCenter for Neuroscience and Behavior Institute of Psychology University of São Paulo (USP)
dc.description.affiliationDepartment of Anatomy Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationElectron Microscopy Center Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Anatomy Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespElectron Microscopy Center Institute of Biosciences São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1111/jne.12723
dc.identifier.citationJournal of Neuroendocrinology.
dc.identifier.doi10.1111/jne.12723
dc.identifier.issn1365-2826
dc.identifier.issn0953-8194
dc.identifier.scopus2-s2.0-85066907157
dc.identifier.urihttp://hdl.handle.net/11449/189222
dc.language.isoeng
dc.relation.ispartofJournal of Neuroendocrinology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjecthypothalamus
dc.subjectmaternal behaviour
dc.subjectmedial preoptic area
dc.subjectneuroplasticity
dc.subjectoxytocin
dc.titleCharacterisation and origins of melanin-concentrating hormone immunoreactive fibres of the posterior lobe of the pituitary and median eminence during lactation in the Long-Evans raten
dc.typeArtigo
unesp.author.orcid0000-0002-2020-2289[3]
unesp.author.orcid0000-0002-8908-6474[10]

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