Interleukin-15 increases Paracoccidioides brasiliensis killing by human neutrophils

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dc.contributor.authorTavian, E. G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:34:28Z
dc.date.available2014-05-20T13:34:28Z
dc.date.issued2008-01-01
dc.description.abstractInterleukin-15 is a pro-inflammatory cytokine produced by a wide range of different cell types, especially monocytes and macrophages, in response to infective agents, playing a crucial and modulatory role in innate and adaptive immune response. Infections by intracellular microorganisms such as some bacteria, protozoa and fungi point out the role of IL-15 in the activation of monocytes/macrophages and neutrophils, a process that represents an important defense mechanism in early periods of infection during the development of innate immune response. The aims of the present study were to evaluate the effects of IL-15 on human neutrophil fungicidal activity against a high virulent Paracoccidioides brasiliensis strain ( Pb18) and to verify whether this activity was mediated by oxidative metabolism such as the production of superoxide anion and H2O2 and if it was associated with an alteration of cytokine ( IL-8 and TNF-alpha) levels. Neutrophils from peripheral blood of healthy individuals were incubated in the presence and absence of IL-15 ( 12.5 - 250ng/ml) for 18h, at 37 degrees C, under tension of 5% CO2, then infected with Pb18 for 4h and evaluated for fungicidal activity, production of superoxide anion and H2O2, and quantification of cytokines IL-8 and TNF-a in the supernatant. Preincubation of neutrophils with IL-15 induced a significant increase in the fungicidal activity of such cells in a dose-dependent manner. After activation, there was an increase in the production of superoxide anion and H2O2 by these cells, suggesting participation of such metabolites in fungicidal activity. Catalase inhibits fungicidal activity, confirming the role of H2O2 in fungus killing. However, the levels of TNF-alpha and IL-8 were not modified after incubation with IL-15, which suggests that its role is not mediated by those cytokines. Taken together, results showed that IL-15 had a modulatory effect on human neutrophils infected in vitro with a high virulent strain of P. brasiliensis, which was characterized by an increased fungicidal activity mediated by a dependent mechanism of oxidative metabolism.en
dc.description.affiliationUNESP, São Paulo State Univ, Dept Trop Dis, Botucatu Sch Med, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, São Paulo State Univ, Dept Trop Dis, Botucatu Sch Med, Botucatu, SP, Brazil
dc.format.extent188-188
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992008000100017
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 14, n. 1, p. 188-188, 2008.
dc.identifier.doi10.1590/S1678-91992008000100017
dc.identifier.fileS1678-91992008000100017.pdf
dc.identifier.issn1678-9199
dc.identifier.scieloS1678-91992008000100017
dc.identifier.urihttp://hdl.handle.net/11449/11814
dc.identifier.wosWOS:000254511600017
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectneutrophilsen
dc.subjectIL-15en
dc.subjectParacoccidioides brasiliensisen
dc.subjectsuperoxide anionen
dc.subjectH2O2en
dc.subjectfungicidal activity.en
dc.titleInterleukin-15 increases Paracoccidioides brasiliensis killing by human neutrophilsen
dc.typeResumo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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