Sperm Organelle Morphologic Abnormalities: Contributing Factors and Effects on Intracytoplasmic Sperm Injection Cycles Outcomes

Página do item simplificado
dc.contributor.authorde Almeida Ferreira Braga, Daniela Paes
dc.contributor.authorSetti, Amanda S.
dc.contributor.authorFigueira, Rita C. S.
dc.contributor.authorNichi, Marcilio
dc.contributor.authorMartinhago, Ciro D.
dc.contributor.authorIaconelli, Assumpto
dc.contributor.authorBorges, Edson
dc.contributor.institutionFertil Assisted Fertilizat Ctr
dc.contributor.institutionSapientiae Inst Educ & Res Ctr Assisted Repr
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionRDO Med Diag
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:35:30Z
dc.date.available2014-05-20T13:35:30Z
dc.date.issued2011-10-01
dc.description.abstractOBJECTIVE To (1) analyze possible relationships between motile sperm organelle morphology examination (MSOME) and sperm chromatin status, aneuploidy incidence, and patient's age; (2) determine the effects of sperm morphologic abnormalities on intracytoplasmic sperm injection (ICSI) outcomes; and (3) identify the benefits of intracytoplasmic morphologically selected sperm injection (IMSI) in patients with high DNA fragmentation rate.METHODS The study was performed in 50 patients undergoing ICSI cycles. The MSOME, sperm DNA fragmentation, and sperm aneuploidy incidence were performed in 200 sperm cells of each patient. Regression models were used to assess the relationships among sperm morphology and sperm aneuploidy, sperm DNA fragmentation, patient's age, and ICSI outcomes. In cycles with patients showing a high incidence of DNA fragmentation, oocytes were split into 2 groups according to the sperm selection method: Standard-ICSI (n = 82) and IMSI (n = 79). Fertilization and high-quality embryo rates were compared between the groups.RESULTS A close relationship between sperm DNA fragmentation and the presence of vacuoles in the MSOME was noted. The patient's age was correlated to the presence of vacuoles. No correlation between sperm aneuploidy and IMSI was observed. Vacuolated cells were negatively correlated with fertilization, pregnancy, and implantation. In patients with a high incidence of sperm DNA fragmentation, fertilization and high-quality embryo rates were similar when comparing IMSI and Standard-ICSI.CONCLUSIONS Our data demonstrate a correlation between paternal age and the incidence of nuclear vacuoles, as well as an effect of large and small vacuoles on late embryo development. UROLOGY 78: 786-791, 2011. (C) 2011 Elsevier B.V.en
dc.description.affiliationFertil Assisted Fertilizat Ctr, São Paulo, Brazil
dc.description.affiliationSapientiae Inst Educ & Res Ctr Assisted Repr, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Anim Reprod, Fac Vet Med, São Paulo, Brazil
dc.description.affiliationRDO Med Diag, São Paulo, Brazil
dc.description.affiliationBotucatu Med Sch Unesp, Dept Obstet & Gynaecol, São Paulo, Brazil
dc.description.affiliationUnespBotucatu Med Sch Unesp, Dept Obstet & Gynaecol, São Paulo, Brazil
dc.format.extent786-791
dc.identifierhttp://dx.doi.org/10.1016/j.urology.2011.06.018
dc.identifier.citationUrology. New York: Elsevier B.V., v. 78, n. 4, p. 786-791, 2011.
dc.identifier.doi10.1016/j.urology.2011.06.018
dc.identifier.issn0090-4295
dc.identifier.urihttp://hdl.handle.net/11449/12219
dc.identifier.wosWOS:000296023000018
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofUrology
dc.relation.ispartofjcr2.300
dc.relation.ispartofsjr1,111
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleSperm Organelle Morphologic Abnormalities: Contributing Factors and Effects on Intracytoplasmic Sperm Injection Cycles Outcomesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0003-4262-0825[4]
unesp.author.orcid0000-0002-7802-8906[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Ginecologia e Obstetrícia - FMB