Transcriptome response signatures associated with the overexpression of a mitochondrial uncoupling protein (AtUCP1) in tobacco

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dc.contributor.authorNunes Laitz, Alessandra Vasconcellos [UNESP]
dc.contributor.authorAcencio, Marcio Luis [UNESP]
dc.contributor.authorBudzinski, Ilara G. F.
dc.contributor.authorLabate, Monica T. V.
dc.contributor.authorLemke, Ney [UNESP]
dc.contributor.authorMartins Ribolla, Paulo Eduardo [UNESP]
dc.contributor.authorMaia, Ivan G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-10-21T13:11:23Z
dc.date.available2015-10-21T13:11:23Z
dc.date.issued2015-06-24
dc.description.abstractMitochondrial inner membrane uncoupling proteins (UCP) dissipate the proton electrochemical gradient established by the respiratory chain, thus affecting the yield of ATP synthesis. UCP overexpression in plants has been correlated with oxidative stress tolerance, improved photosynthetic efficiency and increased mitochondrial biogenesis. This study reports the main transcriptomic responses associated with the overexpression of an UCP (AtUCP1) in tobacco seedlings. Compared to wild-type (WT), AtUCP1 transgenic seedlings showed unaltered ATP levels and higher accumulation of serine. By using RNA-sequencing, a total of 816 differentially expressed genes between the investigated overexpressor lines and the untransformedWT control were identified. Among them, 239 were up-regulated and 577 were down-regulated. As a general response to AtUCP1 overexpression, noticeable changes in the expression of genes involved in energy metabolism and redox homeostasis were detected. A substantial set of differentially expressed genes code for products targeted to the chloroplast and mainly involved in photosynthesis. The overall results demonstrate that the alterations in mitochondrial function provoked by AtUCP1 overexpression require important transcriptomic adjustments to maintain cell homeostasis. Moreover, the occurrence of an important cross-talk between chloroplast and mitochondria, which culminates in the transcriptional regulation of several genes involved in different pathways, was evidenced.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Genet, Botucatu, SP, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Fis &Biofis, Botucatu, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba, SP, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Parasitol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Instituto de Biociências, Departamento de Genética, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Instituto de Biociências, Departamento de Física e Biofísica, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Instituto de Biociências, Departamento de Parasitologia, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2013/12947-2
dc.format.extent1-19
dc.identifierhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0130744
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 10, n. 6, p. 1-19, 2015.
dc.identifier.doi10.1371/journal.pone.0130744
dc.identifier.fileWOS000356932500122.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes7977035910952141
dc.identifier.lattes3577149748456880
dc.identifier.urihttp://hdl.handle.net/11449/128600
dc.identifier.wosWOS:000356932500122
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleTranscriptome response signatures associated with the overexpression of a mitochondrial uncoupling protein (AtUCP1) in tobaccoen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes7977035910952141
unesp.author.lattes3577149748456880
unesp.author.orcid0000-0001-7463-4303[5]
unesp.author.orcid0000-0002-8278-240X[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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