Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts

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dc.contributor.authorHata Viveiros, Magda Massae [UNESP]
dc.contributor.authorSilva, Márcia Guimarães [UNESP]
dc.contributor.authorda Costa, José Galberto Martins
dc.contributor.authorde Oliveira, Anselmo Gomes [UNESP]
dc.contributor.authorRubio, Carolina [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorRainho, Cláudia Aparecida [UNESP]
dc.contributor.authorSchellini, Silvana Artioli [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionRegional University of Cariri
dc.date.accessioned2023-07-29T13:31:43Z
dc.date.available2023-07-29T13:31:43Z
dc.date.issued2022-01-01
dc.description.abstract• AIM: To investigate the anti-inflammatory effects of the sesquiterpenes α-humulene and β-caryophyllene on pterygium fibroblasts. • METHODS: Primary cultures of pterygium fibroblasts were established. Third passage pterygium fibroblasts were exposed to α-humulene and β-caryophyllene separately and together. The cell viability was assessed by 3-(4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide (MTT) assay at 12, 24, 48, and 72h after exposure. The levels of the inflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α and IL-10 in the conditioned culture medium were assessed by enzyme-linked immunosorbent assay (ELISA) at 12, 24 and 48h after exposure. Data were statistically analyzed using Friedman repeated measures analysis of variances on ranks. • RESulTS: The 25 μmol/L β-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure (P=0.041). The levels of IL-1β, IL-8, IL-10, and TNF-α were very low and had no statistically significant variations after exposure to α-humulene, β-caryophyllene, or both compounds together. • COnCluSIOn:Theexposureto25μmol/Lofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after 48h. This sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.en
dc.description.affiliationDepartment of Ophthalmology School of Medicine of Botucatu São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationDepartment of Pathology School of Medicine of Botucatu São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationDepartment of Biological Chemistry Regional University of Cariri, Ceará
dc.description.affiliationDepartment of Pharmacology School of Pharmaceutical Sciences São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationBiological Sciences Institute of Biosciences of Botucatu São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationDepartment of Biostatistics São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationUnespDepartment of Ophthalmology School of Medicine of Botucatu São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationUnespDepartment of Pathology School of Medicine of Botucatu São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationUnespDepartment of Pharmacology School of Pharmaceutical Sciences São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationUnespBiological Sciences Institute of Biosciences of Botucatu São Paulo State University-UNESP, Sao Paulo
dc.description.affiliationUnespDepartment of Biostatistics São Paulo State University-UNESP, Sao Paulo
dc.format.extent1903-1907
dc.identifierhttp://dx.doi.org/10.18240/ijo.2022.12.02
dc.identifier.citationInternational Journal of Ophthalmology, v. 15, n. 12, p. 1903-1907, 2022.
dc.identifier.doi10.18240/ijo.2022.12.02
dc.identifier.issn2227-4898
dc.identifier.issn2222-3959
dc.identifier.scopus2-s2.0-85143542292
dc.identifier.urihttp://hdl.handle.net/11449/247999
dc.language.isoeng
dc.relation.ispartofInternational Journal of Ophthalmology
dc.sourceScopus
dc.subjectcytokines
dc.subjectfibroblasts
dc.subjectpterygium
dc.subjectα-humulene
dc.subjectβ-caryophyllene
dc.titleAnti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblastsen
dc.typeArtigo

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