Determination of acetonitrile and cyanide in rat blood: Application to an experimental study

Página do item simplificado
dc.contributor.authorMateus, Fabiano Henrique
dc.contributor.authorLepera, José Salvador [UNESP]
dc.contributor.authorLanchote, Vera Lucia
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:17Z
dc.date.available2014-05-27T11:21:17Z
dc.date.issued2005-03-01
dc.description.abstractMethods were developed for the analysis of acetonitrile and its metabolite cyanide in the blood of rats exposed to acetonitrile. Acetonitrile was analyzed by the headspace technique coupled to gas chromatography with detection by flame ionization, and cyanide was analyzed by high-performance liquid chromatography with fluorescence detection (λ ex = 418 nm and λ em = 460 nm) after derivatization of the ion with naphthalene 2,3-dicarboxyaldehyde and taurine. The quantitation limits of the methods for the analysis of acetonitrile and cyanide were 4.875 μg/mL and 0.025 μg/mL, respectively. The coefficients of variation of 10% or less obtained for intra- and interassay precision indicate the precision of these analytical methods and the systematic errors, all less than 5%, indicate that the methods are quite accurate. The methods were applied to an experimental study after the animals received acetonitrile at the doses of 2 mmol/kg or 5 mmol/kg.en
dc.description.affiliationFaculdade Cie. Farmaceuticas R. Universidade de São Paulo Depto. Analises Clinicas, T., São Paulo
dc.description.affiliationFaculdade Cie. Farmaceuticas A. Universidade Estadual Paulista Depto. Principios Ativos Naturais T.
dc.description.affiliationFaculdade Cie. Farmaceuticas R. Universidade de São Paulo Depto. Analises Clinicas, T., Avenida do Café s/n, Ribeirão Preto, SP
dc.description.affiliationUnespFaculdade Cie. Farmaceuticas A. Universidade Estadual Paulista Depto. Principios Ativos Naturais T.
dc.format.extent105-109
dc.identifierhttp://jat.oxfordjournals.org/content/29/2/105.long
dc.identifier.citationJournal of Analytical Toxicology, v. 29, n. 2, p. 105-109, 2005.
dc.identifier.issn0146-4760
dc.identifier.lattes6710074203174471
dc.identifier.scopus2-s2.0-15244348958
dc.identifier.urihttp://hdl.handle.net/11449/68162
dc.identifier.wosWOS:000227342900004
dc.language.isoeng
dc.relation.ispartofJournal of Analytical Toxicology
dc.relation.ispartofjcr2.599
dc.relation.ispartofsjr1,065
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subject2,3 naphthalenedicarboxaldehyde
dc.subjectacetonitrile
dc.subjectcyanide
dc.subjection
dc.subjecttaurine
dc.subjectaccuracy
dc.subjectanalytic method
dc.subjectanimal experiment
dc.subjectblood examination
dc.subjectcontrolled study
dc.subjectderivatization
dc.subjectfluorescence
dc.subjectgas chromatography
dc.subjecthigh performance liquid chromatography
dc.subjectmale
dc.subjectnonhuman
dc.subjectrat
dc.subjectAcetonitriles
dc.subjectAnimals
dc.subjectChromatography, High Pressure Liquid
dc.subjectCyanides
dc.subjectFlame Ionization
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReproducibility of Results
dc.titleDetermination of acetonitrile and cyanide in rat blood: Application to an experimental studyen
dc.typeArtigo
unesp.author.lattes6710074203174471
unesp.author.orcid0000-0002-2645-7469[2]
unesp.author.orcid0000-0002-0074-4953[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

Arquivos

Coleções

Artigos - Princípios Ativos Naturais e Toxicologia - FCFAR