Ecological insights to track cytotoxic compounds among maytenus ilicifolia living individuals and clones of an ex situ collection

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dc.contributor.authorPavarini, Daniel Petinatti [UNESP]
dc.contributor.authorSelegato, Denise Medeiros [UNESP]
dc.contributor.authorCastro-Gamboa, Ian [UNESP]
dc.contributor.authorDo Sacramento, Luiz Vitor Silva [UNESP]
dc.contributor.authorFurlan, Maysa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionQueen’s University Belfast
dc.date.accessioned2019-10-06T17:06:32Z
dc.date.available2019-10-06T17:06:32Z
dc.date.issued2019-01-01
dc.description.abstractBiodiversity is key for maintenance of life and source of richness. Nevertheless, concepts such as phenotype expression are also pivotal to understand how chemical diversity varies in a living organism. Sesquiterpene pyridine alkaloids (SPAs) and quinonemethide triterpenes (QMTs) accumulate in root bark of Celastraceae plants. However, despite their known bioactive traits, there is still a lack of evidence regarding their ecological functions. Our present contribution combines analytical tools to study clones and individuals of Maytenus ilicifolia (Celastraceae) kept alive in an ex situ collection and determine whether or not these two major biosynthetic pathways could be switched on simultaneously. The relative concentration of the QMTs maytenin (1) and pristimerin (2), and the SPA aquifoliunin E1 (3) were tracked in raw extracts by HPLC-DAD and 1H-NMR. Hierarchical Clustering Analysis (HCA) was used to group individuals according their ability to accumulate these metabolites. Semi-quantitative analysis showed an extensive occurrence of QMT in most individuals, whereas SPA was only detected in minor abundance in five samples. Contrary to QMTs, SPAs did not accumulate extensively, contradicting the hypothesis of two different biosynthetic pathways operating simultaneously. Moreover, the production of QMT varied significantly among samples of the same ex situ collection, suggesting that the terpene contents in root bark extracts were not dependent on abiotic effects. HCA results showed that QMT occurrence was high regardless of the plant age. This data disproves the hypothesis that QMT biosynthesis was age-dependent. Furthermore, clustering analysis did not group clones nor same-age samples together, which might reinforce the hypothesis over gene regulation of the biosynthesis pathways. Indeed, plants from the ex situ collection produced bioactive compounds in a singular manner, which postulates that rhizosphere environment could offer ecological triggers for phenotypical plasticity.en
dc.description.affiliationInstituto de Química University Estadual Paulista-UNESP, Rua Prof. Francisco Degni, 55, Quitandinha
dc.description.affiliationInstitute for Global Food Security School of Biological Sciences Queen’s University Belfast, Cloreen Park, Malone Road
dc.description.affiliationFaculdade de Ciências Farmacêuticas University Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1
dc.description.affiliationUnespInstituto de Química University Estadual Paulista-UNESP, Rua Prof. Francisco Degni, 55, Quitandinha
dc.description.affiliationUnespFaculdade de Ciências Farmacêuticas University Estadual Paulista-UNESP, Rod. Araraquara-Jaú km 1
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/07600-3
dc.description.sponsorshipIdFAPESP: 2014/05935-0
dc.description.sponsorshipIdFAPESP: 2014/14067-2
dc.description.sponsorshipIdFAPESP: 2017/06466-2
dc.identifierhttp://dx.doi.org/10.3390/molecules24061160
dc.identifier.citationMolecules, v. 24, n. 6, 2019.
dc.identifier.doi10.3390/molecules24061160
dc.identifier.issn1420-3049
dc.identifier.lattes1308042794786872
dc.identifier.scopus2-s2.0-85063569767
dc.identifier.urihttp://hdl.handle.net/11449/190233
dc.language.isoeng
dc.relation.ispartofMolecules
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCelastraceae
dc.subjectChemical diversity
dc.subjectHierarchical Clustering Analysis (HCA)
dc.subjectQuinonemethide triterpenes
dc.subjectSesquiterpene pyridine alkaloids
dc.titleEcological insights to track cytotoxic compounds among maytenus ilicifolia living individuals and clones of an ex situ collectionen
dc.typeArtigo
unesp.author.lattes1308042794786872

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