Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface

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dc.contributor.authorSoares, Diana Gabriela
dc.contributor.authorSacono, Nancy Tomoko
dc.contributor.authorRibeiro, Ana Paula Dias
dc.contributor.authorLeite, Maria Luisa [UNESP]
dc.contributor.authorDuque, Carla Caroline de Oliveira [UNESP]
dc.contributor.authorGallinari, Marjorie De Oliveira
dc.contributor.authorPacheco, Leandro Edgar
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUnip - Universidade Paulista
dc.contributor.institutionUniversity of Florida
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-03-01T20:39:07Z
dc.date.available2023-03-01T20:39:07Z
dc.date.issued2022-01-01
dc.description.abstractThis paper aimed to assess the influence of adhesive restoration interface on the diffusion of hydrogen peroxide (H2O2), indirect toxicity, and pro- inflammatory mediators expression by odontoblast-like cells, after in-office tooth whitening. Dental cavities prepared in bovine enamel/dentin discs were adhesively restored and subjected or not to hydrolytic degradation (HD). A whitening gel with 35% ^CMWG) was applied for 45 min onto restored and non-restored specimens adapted to artificial pulp chambers giving rise to the groups: SD- intact discs (control); SD/HP- whitened intact discs; RT/HP- restored and whitened discs; and RT/HD/HP- restored and whitened discs subjected to HD. The extracts (culture medium + WG components diffused through enamel/dentin/restoration interface) were collected and applied to odontoblast-like MDPC-23 cells. The study evaluated the amount of H2O2 in the extracts, as well as the cell viability (CV), cell morphology (CM), and gene expression of inflammatory mediators (TNF-a and COX-2) by the pulp cells exposed to the extracts (ANOVA and Tukey tests; 5% significance). All whitened groups presented lower CV than SD (control; p<0.05). The highest CV reduction and gene expression of TNF-a and COX-2 was observed in the RT/HD/HP group in comparison with SD/HP and RT/HP (control; p<0.05). CM alterations occurred in all whitened groups. The intensity of these cell side effects was directly related with the amount of H2O2 in the extracts. We concluded that adhesive restoration of dental cavity increases the H2O2 diffusion after in-office whitening, enhancing the indirect toxicity of this therapy and trigger pro-inflammatory overexpression by MDPC-23 cells.en
dc.description.affiliationEndodontics and Dental Materials Bauru School of Dentistry University of Sao Paulo, SP
dc.description.affiliationDepartment of Pediatric Dentistry Unip - Universidade Paulista, Br 153 Highway, Km 503, area 1-5, s/n - Fazenda Botafogo
dc.description.affiliationDepartment of Restorative Dental Sciences College of Dentistry University of Florida, 1395 Center Drive
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual Paulista
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Sao Paulo State University
dc.description.affiliationDepartment of Physiology and Pathology Sao Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry Universidade Estadual Paulista
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry Sao Paulo State University
dc.description.affiliationUnespDepartment of Physiology and Pathology Sao Paulo State University (UNESP) School of Dentistry
dc.format.extent83-90
dc.identifierhttp://dx.doi.org/10.1590/0103-6440202204688
dc.identifier.citationBrazilian Dental Journal, v. 33, n. 2, p. 83-90, 2022.
dc.identifier.doi10.1590/0103-6440202204688
dc.identifier.issn1806-4760
dc.identifier.issn0103-6440
dc.identifier.scopus2-s2.0-85129393366
dc.identifier.urihttp://hdl.handle.net/11449/240929
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.sourceScopus
dc.subjectAdhesion
dc.subjectInflammation
dc.subjectOdontoblasts
dc.subjectTooth-bleaching
dc.subjectToxicity
dc.titlePro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surfaceen
dc.typeArtigo
unesp.author.orcid0000-0002-1485-6104[1]
unesp.author.orcid0000-0002-4514-3588[2]
unesp.author.orcid0000-0003-0840-4205[3]
unesp.author.orcid0000-0001-6650-3637[4]
unesp.author.orcid0000-0002-5898-4132[5]
unesp.author.orcid0000-0002-1804-9625[6]
unesp.author.orcid0000-0001-7545-1760[7]
unesp.author.orcid0000-0002-2846-2325[8]
unesp.author.orcid0000-0002-7455-6867[9]

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Artigos - Materiais Odontológicos e Prótese - FOAR
Artigos - Clínica Infantil - FOAR