Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line

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dc.contributor.authorAranha, Andreza M.F. [UNESP]
dc.contributor.authorGiro, Elisa M.A. [UNESP]
dc.contributor.authorSouza, Pedro P.C. [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorde Souza Costa, Carlos A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:57Z
dc.date.available2014-05-27T11:21:57Z
dc.date.issued2006-09-01
dc.description.abstractObjective: The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23). Methods: Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers' recommended time (MRT = 30 s), under-cured (0.5 MRT = 15 s), over-cured (1.5 MRT = 45 s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5 μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000 cells/cm2) were plated in each well and incubated for 72 h in a humidified incubator at 37 °C with 5% CO2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). Results: Fuji Lining LC was less cytotoxic than Vitrebond (p < 0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45 s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23. Significance: The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer' recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells. © 2005 Academy of Dental Materials.en
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry School of Dentistry University of São Paulo State - UNESP, Araraquara, São Paulo
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry University of São Paulo State - UNESP, Araraquara, São Paulo
dc.description.affiliationUnespDepartment of Orthodontics and Pediatric Dentistry School of Dentistry University of São Paulo State - UNESP, Araraquara, São Paulo
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry University of São Paulo State - UNESP, Araraquara, São Paulo
dc.format.extent864-869
dc.identifierhttp://dx.doi.org/10.1016/j.dental.2005.11.015
dc.identifier.citationDental Materials, v. 22, n. 9, p. 864-869, 2006.
dc.identifier.doi10.1016/j.dental.2005.11.015
dc.identifier.issn0109-5641
dc.identifier.lattes4517484241515548
dc.identifier.scopus2-s2.0-33746635567
dc.identifier.urihttp://hdl.handle.net/11449/69053
dc.language.isoeng
dc.relation.ispartofDental Materials
dc.relation.ispartofjcr4.039
dc.relation.ispartofsjr2,106
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCuring regime
dc.subjectCytotoxicity
dc.subjectGlass-ionomer cements
dc.subjectHEMA
dc.subjectOdontoblast
dc.subjectCell culture
dc.subjectChemical bonds
dc.subjectCuring
dc.subjectGlass
dc.subjectIonomers
dc.subjectMorphology
dc.subjectDental cement
dc.subject2 hydroxyethyl methacrylate
dc.subject4 anisyltetrazolium blue
dc.subject4-anisyltetrazolium blue
dc.subjectcoloring agent
dc.subjectdentin bonding agent
dc.subjectFuji glass ionomer lining cement
dc.subjectFuji glass-ionomer lining cement
dc.subjectglass ionomer
dc.subjectmethacrylic acid derivative
dc.subjectresin
dc.subjecttetrazolium
dc.subjectVitrabond
dc.subjectanimal
dc.subjectcell line
dc.subjectcell shape
dc.subjectchemistry
dc.subjectdental surgery
dc.subjectdrug effect
dc.subjectlight
dc.subjectmetabolism
dc.subjectmouse
dc.subjectodontoblast
dc.subjectphase transition
dc.subjectradiation exposure
dc.subjectscanning electron microscopy
dc.subjectAnimals
dc.subjectCell Line, Transformed
dc.subjectCell Shape
dc.subjectColoring Agents
dc.subjectDental Cavity Lining
dc.subjectDentin-Bonding Agents
dc.subjectGlass Ionomer Cements
dc.subjectLight
dc.subjectMethacrylates
dc.subjectMice
dc.subjectMicroscopy, Electron, Scanning
dc.subjectOdontoblasts
dc.subjectPhase Transition
dc.subjectResins, Synthetic
dc.subjectTetrazolium Salts
dc.titleEffect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell lineen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-7455-6867[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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