Crystal structure of a dimeric Ser49 PLA(2)-like myotoxic component of the Vipera ammodytes meridionalis venomics reveals determinants of myotoxicity and membrane damaging activity
dc.contributor.author | Georgieva, Dessislava | |
dc.contributor.author | Coronado, Monika | |
dc.contributor.author | Oberthuer, Dominik | |
dc.contributor.author | Buck, Friedrich | |
dc.contributor.author | Duhalov, Deyan | |
dc.contributor.author | Arni, Raghuvir K. [UNESP] | |
dc.contributor.author | Betzel, Christian [UNESP] | |
dc.contributor.institution | Univ Hamburg | |
dc.contributor.institution | Bulgarian Acad Sci | |
dc.contributor.institution | Univ Med Ctr Hamburg Eppendorf | |
dc.contributor.institution | BUL BIO NCIPD Ltd | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T14:02:31Z | |
dc.date.available | 2014-05-20T14:02:31Z | |
dc.date.issued | 2012-01-01 | |
dc.description.abstract | Myotoxicity and membrane damage play a central role in the life-threatening effects of the viper envenomation. Myotoxins are an important part of the viper venomics. A Ser49 PLA(2)-like myotoxin from the venom of Vipera ammodytes meridionalis, the most venomous snake in Europe, was crystallized and its three-dimensional structure determined. The toxin is devoid of phospholipolytic activity. The structure demonstrates a formation of dimers. In the dimers functionally important peptide segments, located on the protein surface, point in the same direction which can strengthen the pharmacological effect. This supports the hypothesis about the physiological importance of the toxin oligomerization for the myotoxicity and membrane damage. The crystallographic model revealed that the structural determinants of myotoxicity (a positively charged C-terminal region and a hydrophobic knuckle) are fully exposed on the protein surface and accessible for interactions with target membranes. Distortion of the catalytic site region explains the absence of enzymatic activity. The structure reveals anion-binding sites which can be considered as possible sites of interactions of the toxin with a negatively charged membrane surface. The high structural similarity of the Ser49 myotoxin and Asp49 PLA(2) from the same venom suggests an evolutionary relationship: probably, the Ser49 myotoxin is a product of evolution of the catalytically active phospholipase A(2). The first toxin lost the enzymatic activity which is not necessary for the myotoxicity but preserved the cytotoxicity and membrane damaging activity as important components of the venom toxicity. | en |
dc.description.affiliation | Univ Hamburg, Lab Struct Biol Infect & Inflammat, DESY, Inst Biochem & Mol Biol, D-22603 Hamburg, Germany | |
dc.description.affiliation | Bulgarian Acad Sci, Inst Organ Chem, BU-1113 Sofia, Bulgaria | |
dc.description.affiliation | Univ Med Ctr Hamburg Eppendorf, Inst Clin Chem, D-20246 Hamburg, Germany | |
dc.description.affiliation | BUL BIO NCIPD Ltd, Sofia, Bulgaria | |
dc.description.affiliation | Univ Estadual Paulista UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | |
dc.description.sponsorship | Deutsche Forschungsgemeinschaft (DFG) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorship | Deutscher Akademischer Austauschdienst (DAAD) | |
dc.description.sponsorship | Bulgarian National Foundation for Scientific Research | |
dc.description.sponsorshipId | DFG: BE 1443-18-1 | |
dc.description.sponsorshipId | CAPES: 80563/2911 | |
dc.description.sponsorshipId | DAAD (PROBAL): 50754442 | |
dc.description.sponsorshipId | Bulgarian National Foundation for Scientific Research: TK-B-1610/06 | |
dc.format.extent | 1405-1411 | |
dc.identifier | http://dx.doi.org/10.1039/c2mb05490f | |
dc.identifier.citation | Molecular Biosystems. Cambridge: Royal Soc Chemistry, v. 8, n. 5, p. 1405-1411, 2012. | |
dc.identifier.doi | 10.1039/c2mb05490f | |
dc.identifier.issn | 1742-206X | |
dc.identifier.lattes | 9162508978945887 | |
dc.identifier.orcid | 0000-0003-2460-1145 | |
dc.identifier.uri | http://hdl.handle.net/11449/22037 | |
dc.identifier.wos | WOS:000302368600003 | |
dc.language.iso | eng | |
dc.publisher | Royal Soc Chemistry | |
dc.relation.ispartof | Molecular Biosystems | |
dc.relation.ispartofjcr | 2.759 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Crystal structure of a dimeric Ser49 PLA(2)-like myotoxic component of the Vipera ammodytes meridionalis venomics reveals determinants of myotoxicity and membrane damaging activity | en |
dc.type | Artigo | |
dcterms.license | http://www.rsc.org/AboutUs/Copyright/Authordeposition.asp | |
dcterms.rightsHolder | Royal Soc Chemistry | |
unesp.author.lattes | 9162508978945887[6] | |
unesp.author.orcid | 0000-0003-2460-1145[6] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto | pt |
Arquivos
Licença do Pacote
1 - 2 de 2
Nenhuma Miniatura disponível
- Nome:
- license.txt
- Tamanho:
- 1.71 KB
- Formato:
- Item-specific license agreed upon to submission
- Descrição:
Nenhuma Miniatura disponível
- Nome:
- license.txt
- Tamanho:
- 1.71 KB
- Formato:
- Item-specific license agreed upon to submission
- Descrição: