Biochemical responses, morphometric changes, genotoxic effects and CYP1A expression in the armored catfish Pterygoplichthys anisitsi after 15 days of exposure to mineral diesel and biodiesel

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dc.contributor.authorFelicio, Andreia Arantes [UNESP]
dc.contributor.authorMartins Parente, Thiago Estevam
dc.contributor.authorMaschio, Lucilene Regina [UNESP]
dc.contributor.authorNogueira, Lilian [UNESP]
dc.contributor.authorRodrigues Venancio, Larissa Paola [UNESP]
dc.contributor.authorRebelo, Mauro de Freitas
dc.contributor.authorSchlenk, Daniel
dc.contributor.authorAlmeida, Eduardo Alves de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversity of California
dc.date.accessioned2015-10-21T13:14:47Z
dc.date.available2015-10-21T13:14:47Z
dc.date.issued2015-05-01
dc.description.abstractDespite being considered friendlier to the environment, biodiesel fuel can be harmful to aquatic organisms, especially when combined with petroleum diesel fuel. In this work we evaluated the effects of mineral diesel fuel containing increasing concentrations of biodiesel (5% and 20%, namely B5 and B20) and pure biodiesel (B100), at concentrations of 0.001 and 0.01 mL L-1, after 15 days of exposure, in armored catfish (Pterygoplichtys anisitsi). Toxicity tests were also performed to estimate LC50 values (96 h) for each compound. Biotransformation enzymes [ethoxyresorufin-O-deethylase (EROD), and glutathione S-transferase (GST)] as well as oxidative stress markers (superoxide dismutase, SOD, catalase, CAT, glutathione peroxidase, GPx, and the level of lipid peroxidation) were measured in liver and gills after treatment. Genotoxic effects were also accessed in erythrocytes using the comet assay and by evaluating the frequency of micronuclei formation. Further, the mRNA of cytochrome P450 1A (CYP1A) was also measured in liver. Mortality was not observed even exposure to concentrations as high as 6.0 mL L-1. EROD and GST activities were increased after B5 and B20 treatments; however, CYP1A mRNA induction was not observed. SOD and CAT activities were decreased, but GPx was significantly higher for all treatments in gills. There were no significant changes in lipid peroxidation, but genotoxicity markers revealed that all treatments increased comet scores. Fuels B5 and B20 increased micronuclei frequency. Our results indicate that despite being less toxic, biodiesel may cause sublethal alterations in fish that may alter long term health. (c) 2015 Elsevier Inc. All rights reserved.en
dc.description.affiliationLaboratório de Biologia Molecular Ambiental-BioMA, Instituto de Biofísica Carlos Chagas Filho-IBCCF, Universidade Federal do Rio de Janeiro-UFRJ, Brazil
dc.description.affiliationCentro de Estudo de Quelônios (CEQ) e Laboratório de Hemoglobinas de Genética das Doenças Hematológicas (LHGDH), IBILCE, UNESP, São José do Rio Preto, SP, Brasil
dc.description.affiliationDepartment of Environmental Sciences, University of California, Riverside, 3401Watkins Dr, Riverside, CA 92521, USA
dc.description.affiliationUnespUniversidade Estadual Paulista, Centro de Estudo de Quelônios (CEQ) e Laboratório de Hemoglobinas de Genética das Doenças Hematológicas (LHGDH), IBILCE, UNESP, São José do Rio Preto, SP, Brasil, Departamento de Química e Ciências Ambientais, IBILCE, UNESP, São José do Rio Preto, SP, Brasil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2008/58032-7
dc.format.extent26-32
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0147651315000445
dc.identifier.citationEcotoxicology And Environmental Safety. San Diego: Academic Press Inc Elsevier Science, v. 115, p. 26-32, 2015.
dc.identifier.doi10.1016/j.ecoenv.2015.01.034
dc.identifier.issn0147-6513
dc.identifier.lattes6713400866382255
dc.identifier.urihttp://hdl.handle.net/11449/128871
dc.identifier.wosWOS:000353073200005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEcotoxicology And Environmental Safety
dc.relation.ispartofjcr3.974
dc.relation.ispartofsjr1,201
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiodieselen
dc.subjectOxidative stressen
dc.subjectGenotoxicityen
dc.subjectCYP1Aen
dc.subjectBiotransformation enzymeen
dc.subjectCatfishen
dc.titleBiochemical responses, morphometric changes, genotoxic effects and CYP1A expression in the armored catfish Pterygoplichthys anisitsi after 15 days of exposure to mineral diesel and biodieselen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6713400866382255
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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