Antimicrobial activity of water-soluble tetra-cationic porphyrins on Pseudomonas aeruginosa

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dc.contributor.authorUrquhart, Carolina Gonzalez
dc.contributor.authorPinheiro, Ticiane da Rosa
dc.contributor.authorda Silva, Jean Lucas Gutknecht
dc.contributor.authorLeal, Daniela Bitencourt Rosa
dc.contributor.authorBurgo, Thiago Augusto Lima [UNESP]
dc.contributor.authorIglesias, Bernardo Almeida
dc.contributor.authorSantos, Roberto Christ Vianna
dc.contributor.institutionUniversidade Federal de Santa Maria
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:43:36Z
dc.date.available2023-07-29T13:43:36Z
dc.date.issued2023-06-01
dc.description.abstractThis manuscript presents the cytotoxicity, antimicrobial activity, antibiofilm preliminary properties, and associated therapy with commercial drugs using water-soluble tetra-cationic porphyrins against Pseudomonas aeruginosa. Two commercial tetra-cationic porphyrins were tested against a standard strain of P. aeruginosa 01 (PA01) in antibacterial activity assays under dark conditions and irradiated with white light for 120 min. Porphyrin 4-H2TMePor showed better antimicrobial activity and was chosen for further tests. Increased minimum inhibitory concentration was observed in the presence of reactive oxygen species, suggesting that photooxidation was mediated by the singlet oxygen production. In the time-kill curve assay, 4-H2TMePor inhibited bacterial growth in 90 min of irradiation. The checkerboard assay revealed synergistic interactions. Biofilms of the standard PA01 strain and three clinical isolates were formed. The biofilm destruction assay was more efficient for PA01, significantly reducing the biofilm biomass formed compared to the positive control. The associated treatment to destroy the biofilm potentiated a significant decrease in the biofilm biomass compared to the positive control. The photosensitizer did not damage human keratinocytes or mouse fibroblasts in the cytotoxicity assays, demonstrating the safety of using 4-H2TMePor. Atomic force microscopy indicated lower adhesion force, higher cell wall deformation, and higher dissipation energy in the treated control compared to untreated PA01. Given our findings, it is evident that water-soluble tetra-cationic porphyrins have excellent antimicrobial and a preliminary antibiofilm activity against Gram-negative bacteria, proving to be a potential photosensitizer for clinical use.en
dc.description.affiliationPrograma de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde Departamento de Microbiologia e Parasitologia (LAPEMICRO) Universidade Federal de Santa Maria, RS
dc.description.affiliationLaboratório de Imunologia Experimental e Aplicada (LABIBIO) Universidade Federal de Santa Maria (UFSM), RS
dc.description.affiliationDepartment of Chemistry and Environmental Sciences Ibilce Sao Paulo State University (Unesp), R. Cristovao Colombo, 2265, SP
dc.description.affiliationLaboratório de Bioinorgânica e Materiais Porfirínicos (LBMP) Departamento de Química Universidade Federal de Santa Maria (UFSM), RS
dc.description.affiliationUnespDepartment of Chemistry and Environmental Sciences Ibilce Sao Paulo State University (Unesp), R. Cristovao Colombo, 2265, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
dc.description.sponsorshipIdFAPESP: 2014/50906-9
dc.description.sponsorshipIdFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul: PQ-Gaúcho 21/2551-0002114-4
dc.description.sponsorshipIdFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul: PqG – 17/2551-0001075-2
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2022.103266
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 42.
dc.identifier.doi10.1016/j.pdpdt.2022.103266
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-85148909322
dc.identifier.urihttp://hdl.handle.net/11449/248421
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.sourceScopus
dc.subjectAntibiofilm activity
dc.subjectCheckerboard
dc.subjectGram-negative
dc.subjectPhotodynamic therapy
dc.subjectPhotoinactivation
dc.titleAntimicrobial activity of water-soluble tetra-cationic porphyrins on Pseudomonas aeruginosaen
dc.typeArtigo

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