Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro

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dc.contributor.authorPereira, Cristiane Aparecida [UNESP]
dc.contributor.authorCosta, Anna Carolina Borges Pereira [UNESP]
dc.contributor.authorCarreira, Claudia Moura
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorJorge, Antonio Olavo Cardoso [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEscola Superior da Amazônia (ESAMAZ)
dc.date.accessioned2014-05-27T11:29:01Z
dc.date.available2014-05-27T11:29:01Z
dc.date.issued2013-05-01
dc.description.abstractThe purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using erythrosine (ER) and Rose Bengal (RB) photosensitizers and a blue light-emitting diode (LED) on the viability of Streptococcus mutans and Streptococcus sanguinis biofilms. Biofilms were grown in acrylic disks immersed in broth to production of biofilms, inoculated with microbial suspension (106 cells/mL) and incubated for 48 h. After the formation of biofilms, the effects of the photosensitizers ER and RB at a concentration of 5 μM for 5 min and blue LED (455 ± 20 nm) for 180 s, photosensitizers alone and conjugated were evaluated. Next, the disks were placed in tubes with sterile physiological solution (0.9 % sodium chloride) and sonicated for to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in brain heart infusion agar which were then incubated for 48 h. Then the numbers colony-forming units per milliliter (CFU/mL; log 10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by both photosensitizers. The reductions with RB and ER were, 0.62 and 0.52 log10 CFU mL -1 for S. mutans biofilms (p = 0.001), and 0.95 and 0.88 log 10 CFU mL-1 for S. sanguinis biofilms (p = 0.001), respectively. The results showed that biofilms formed in vitro by S. mutans and S. sanguinis, were sensitive to PDI using a blue LED associated with photosensitizers ER or RB, indicating its use in the control of caries and periodontal diseases. © 2012 Springer-Verlag London Ltd.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis School of Dentistry of São José Dos Campos Univ Estadual Paulista (UNESP), Francisco José Longo 777, São Dimas, São José dos Campos 12245-000 SP
dc.description.affiliationDepartment of Dentistry Escola Superior da Amazônia (ESAMAZ), São Pedro, 544, Campina, Belém 66023-570 PA
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis School of Dentistry of São José Dos Campos Univ Estadual Paulista (UNESP), Francisco José Longo 777, São Dimas, São José dos Campos 12245-000 SP
dc.format.extent859-864
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1175-3
dc.identifier.citationLasers in Medical Science, v. 28, n. 3, p. 859-864, 2013.
dc.identifier.doi10.1007/s10103-012-1175-3
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.lattes0322020541055900
dc.identifier.lattes0053567153623569
dc.identifier.scopus2-s2.0-84880724852
dc.identifier.urihttp://hdl.handle.net/11449/75198
dc.identifier.wosWOS:000318184600021
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBiofilm
dc.subjectPhotodynamic inactivation
dc.subjectStreptococccus sanguinis
dc.subjectStreptococcus mutans
dc.subjectagar
dc.subjecterythrosine
dc.subjectphotosensitizing agent
dc.subjectrose bengal
dc.subjectbacterial count
dc.subjectbacterial viability
dc.subjectbacterium culture
dc.subjectbiofilm
dc.subjectblue light
dc.subjectcolony forming unit
dc.subjectcontrolled study
dc.subjectdilution
dc.subjectin vitro study
dc.subjectlight emitting diode
dc.subjectnonhuman
dc.subjectphotodynamic inactivation
dc.subjectphotodynamics
dc.subjectpriority journal
dc.subjectreduction
dc.subjectStreptococcus sanguinis
dc.subjectsuspension cell culture
dc.subjectultrasound
dc.titlePhotodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitroen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes0322020541055900
unesp.author.lattes0053567153623569[5]
unesp.author.orcid0000-0002-1747-6158[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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