Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation

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dc.contributor.authorMendes Soares, Igor Paulino [UNESP]
dc.contributor.authorAnselmi, Caroline [UNESP]
dc.contributor.authorGuiné, Isabela [UNESP]
dc.contributor.authorFernandes, Lídia de Oliveira [UNESP]
dc.contributor.authorPires, Maria Luiza Barucci Araujo [UNESP]
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.authorScheffel, Débora Lopes Salles
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2023-03-02T08:37:44Z
dc.date.available2023-03-02T08:37:44Z
dc.date.issued2022-09-01
dc.description.abstractObjectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Dentistry Maringá State University (UEM), Paraná
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Genetics Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 138984/2020–4
dc.description.sponsorshipIdFAPESP: 2019/16473–1
dc.description.sponsorshipIdCNPq: 303391/2019–7
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2022.104237
dc.identifier.citationJournal of Dentistry, v. 124.
dc.identifier.doi10.1016/j.jdent.2022.104237
dc.identifier.issn0300-5712
dc.identifier.scopus2-s2.0-85134944888
dc.identifier.urihttp://hdl.handle.net/11449/242084
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.sourceScopus
dc.subjectCollagen
dc.subjectDentin
dc.subjectHydroxyproline
dc.subjectMMP
dc.subjectPre-reacted glass filler
dc.subjectProteolysis
dc.titleInhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradationen
dc.typeArtigo

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