Genome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffea

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dc.contributor.authordos Santos, Tiago Benedito
dc.contributor.authorLima, Joni Esrom
dc.contributor.authorFelicio, Mariane Silva
dc.contributor.authorSoares, João Danillo Moura
dc.contributor.authorDomingues, Douglas Silva [UNESP]
dc.contributor.institutionInstituto Agronômico do Paraná
dc.contributor.institutionUniversidade do Oeste Paulista (UNOESTE)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:11:25Z
dc.date.available2018-12-11T17:11:25Z
dc.date.issued2017-01-01
dc.description.abstractNitrogen (N) is quantitatively the main nutrient required by coffee plants, with acquisition mainly by the roots and mostly exported to coffee beans. Nitrate (NO3 -) and ammonium (NH4 +) are the most important inorganic sources for N uptake. Several N transporters encoded by different gene families mediate the uptake of these compounds. They have an important role in source preference for N uptake in the root system. In this study, we performed a genome- wide analysis, including in silico expression and phylogenetic analyses of AMT1, AMT2, NRT1/PTR, and NRT2 transporters in the recently sequenced Coffea canephora genome. We analyzed the expression of six selected transporters in Coffea arabica roots submitted to N deficiency. N source preference was also analyzed in C. arabica using isotopes. C. canephora N transporters follow the patterns observed for most eudicots, where each member of theAMT andNRT families has a particular role in N mobilization, and where some of these are modulated by N deficiency. Despite the prevalence of putative nitrate transporters in the Coffea genome, ammonium was the preferential inorganic N source for N-starved C. arabica roots. This data provides an important basis for fundamental and applied studies to depict molecular mechanisms involved in N uptake in coffee trees.en
dc.description.affiliationLaboratório de Biotecnologia Vegetal Instituto Agronômico do Paraná
dc.description.affiliationUniversidade do Oeste Paulista (UNOESTE)
dc.description.affiliationDepartamento de Botânica Instituto de Ciências Biológicas Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationCentro de Energia Nuclear na Agricultura (CENA) Escola Superior de Agricultura Luiz de Queiroz (ESALQ) Universidade de São Paulo (USP)
dc.description.affiliationDepartamento de Botânica Instituto de Biociências de Rio Claro Universidade Estadual Paulista Júlio Mesquita Filho (UNESP)
dc.description.affiliationUnespDepartamento de Botânica Instituto de Biociências de Rio Claro Universidade Estadual Paulista Júlio Mesquita Filho (UNESP)
dc.format.extent346-359
dc.identifierhttp://dx.doi.org/10.1590/1678-4685-gmb-2016-0041
dc.identifier.citationGenetics and Molecular Biology, v. 40, n. 1, p. 346-359, 2017.
dc.identifier.doi10.1590/1678-4685-gmb-2016-0041
dc.identifier.fileS1415-47572017000200346.pdf
dc.identifier.issn1678-4685
dc.identifier.issn1415-4757
dc.identifier.scieloS1415-47572017000200346
dc.identifier.scopus2-s2.0-85018316708
dc.identifier.urihttp://hdl.handle.net/11449/174495
dc.language.isoeng
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCoffee
dc.subjectGene family evolution
dc.subjectNitrogen transport
dc.subjectNitrogen uptake
dc.titleGenome-wide identification, classification and transcriptional analysis of nitrate and ammonium transporters in Coffeaen
dc.typeArtigo

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