An impedimetric biosensor to test neat serum for dengue diagnosis

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dc.contributor.authorCecchetto, Juliana [UNESP]
dc.contributor.authorCarvalho, Fernanda C. [UNESP]
dc.contributor.authorSantos, Adriano [UNESP]
dc.contributor.authorFernandes, Flavio C. B. [UNESP]
dc.contributor.authorBueno, Paulo R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T20:20:48Z
dc.date.available2015-10-21T20:20:48Z
dc.date.issued2015-07-05
dc.description.abstractNS1, a non-structural dengue protein, has been used as a biomarker for the detection of viremia in dengue diagnosis. In this work, an impedimetric label-free immunosensor based on an anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, a mixed self-assembled monolayer consisting of 11-mercaptoundecanoic acid (for covalent anti-NS1 attachment) and 6-mercaptohexanol (as a spacer) was prepared. The impedance spectra were recorded in the presence of a redox probe ( [Fe(CN)(6)](3-/4-)) to monitor changes in charge-transfer resistance associated with target binding both in PBS and neat serum. A calibration curve was constructed in order to obtain analytical parameters. NS1 was diluted in PBS to final concentrations in the linear range of 0.01-2.00 mu g mL(-1) with a sensitivity of 14.1 percentage decade(-1) (R similar to 0.99) and a limit of detection (LOD) of 3 ng mL(-1). A linear range 0.01-1.00 mu g mL(-1) of NS1 diluted in neat serum was also obtained (R similar to 0.98) with a sensitivity of 10.4 percentage decade(-1) and a LOD of 30 ng mL(-1). The results open new insights into the potential use of this biosensor for point-of-care and bedside applications for dengue diagnosis.en
dc.description.affiliationSão Paulo State University (UNESP) - Department of Physical Chemistry, Chemistry Institute, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State University (UNESP) - Department of Physical Chemistry, Chemistry Institute, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2012-22820-7
dc.format.extent150-154
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0925400515002427
dc.identifier.citationSensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 213, p. 150-154, 2015.
dc.identifier.doi10.1016/j.snb.2015.02.068
dc.identifier.issn0925-4005
dc.identifier.urihttp://hdl.handle.net/11449/129089
dc.identifier.wosWOS:000352152500020
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofSensors And Actuators B-chemical
dc.relation.ispartofjcr5.667
dc.relation.ispartofsjr1,406
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDengueen
dc.subjectNS1 proteinen
dc.subjectElectrochemical impedance spectroscopy (EIS)en
dc.subjectLabel-free biosensoren
dc.subjectBlood serumen
dc.titleAn impedimetric biosensor to test neat serum for dengue diagnosisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0003-2827-0208[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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