Heterologous microsatellite primer pairs informative for the whole genus Arachis

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dc.contributor.authorHoshino, Andrea Akemi [UNESP]
dc.contributor.authorBravo, Juliana Pereira [UNESP]
dc.contributor.authorAngelici, Carla M.L.C.D. [UNESP]
dc.contributor.authorBarbosa, Andréa V. Gobbi [UNESP]
dc.contributor.authorLopes, Catalina Romero [UNESP]
dc.contributor.authorGimenes, Marcos A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Agronômico (IAC)
dc.date.accessioned2014-05-20T13:50:16Z
dc.date.available2014-05-20T13:50:16Z
dc.date.issued2006-01-01
dc.description.abstractThe genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that heterologous primers were very useful in Arachis since they had high transferability among the species (91%) and allowed the amplification of very polymorphic putative loci, which allowed both the characterization of most accessions and to make inferences about the mating systems of some species analyzed. Our data also revealed that the germplasm analyzed showed high variability, even when represented by few accessions.en
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Departamento de Genética
dc.description.affiliationInstituto Agronômico de Campinas Centro de Pesquisa e Desenvolvimento de Recursos Genéticos e Vegetais
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Departamento de Genética
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent665-675
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572006000400016
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 4, p. 665-675, 2006.
dc.identifier.doi10.1590/S1415-47572006000400016
dc.identifier.fileS1415-47572006000400016.pdf
dc.identifier.issn1415-4757
dc.identifier.lattes6903215575686010
dc.identifier.scieloS1415-47572006000400016
dc.identifier.urihttp://hdl.handle.net/11449/17948
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectpeanuten
dc.subjectgenetic relationshipen
dc.subjectmolecular markersen
dc.subjectwild relativesen
dc.titleHeterologous microsatellite primer pairs informative for the whole genus Arachisen
dc.typeArtigo
unesp.author.lattes6903215575686010
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Genética - IBB