HSPA1A, HSPA1L and TRAP1 heat shock genes may be associated with prognosis in ovarian epithelial cancer

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dc.contributor.authorDe Andrade, Warne Pedro [UNESP]
dc.contributor.authorBraga, Leticia Da Conceicao [UNESP]
dc.contributor.authorGoncales, Nikole Gontijo
dc.contributor.authorSilva, Luciana Maria
dc.contributor.authorDa Silva Filho, Agnaldo Lopes [UNESP]
dc.contributor.institutionVera Cruz Hosp
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEzequiel Dias Fdn
dc.contributor.institutionMinas Gerais Fed Univ
dc.date.accessioned2020-12-10T19:47:10Z
dc.date.available2020-12-10T19:47:10Z
dc.date.issued2020-01-01
dc.description.abstractEpithelial ovarian cancer (EOC) is the most lethal gynecological malignancy, with the presence of chemoresistance contributing to the poor prognosis. Heat Shock Proteins (HSPs) genes are activated in response to pathophysiological stress and serve a role in a variety of stages in carcinogenesis, acting primarily as anti-apoptotic agents and in chemotherapy resistance in a variety of tumor types. The current study evaluated the HSP gene expression profile in women with ovarian cancer (OC) and their correlation with clinical and pathological aspects of patients with OC. A total of 51 patients included in the current study were divided into four groups: Primary Epithelial Ovarian Cancer (EOC; n=14), metastatic EOC (n=11), ovarian serous cystadenoma (n=7) and no evidence of ovarian malignancy or control groups (n=19). RNA extraction and reverse transcription-quantitative (RT-q) PCR was then performed on the samples obtained. RT-qPCR was performed to compare TNF receptor associated protein 1 (TRAP]), heat shock protein family (HSP) HSPB1, HSPD1, HSPA1A and HSPA1L expression in primary and metastatic EOCs. TRAP], HSPB1, HSPD1, HSPA1A and HSPAlL gene expression did not differ among groups. HSPA1A, HSPA]L and TRAP] were revealed to be underexpressed in the primary and metastatic EOC groups, with HSPA1L exhibiting the lowest expression. TRAP] expression was higher in tumors at stages I/II compared with those at stages III/IV. No correlation was exhibited between HSP expression and age, menarche, menopause, parity, period after menopause initiation, cytoreduction, CA-125 or overall and disease-free survival. HSPA1A was negatively correlated with the risk of mortality from OC. The results indicated that the downregulation of HSPA1A, HSPA1L and TRAP] could be associated with the clinical prognostic features of women with EOC.en
dc.description.affiliationVera Cruz Hosp, Oncol Serv, BR-30180090 Belo Horizonte, MG, Brazil
dc.description.affiliationSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, Brazil
dc.description.affiliationEzequiel Dias Fdn, Res & Dev Dept, Cellular Biol Serv, BR-30510010 Belo Horizonte, MG, Brazil
dc.description.affiliationMinas Gerais Fed Univ, Sch Med, Dept Obstet & Gynecol, 190 Alfredo Balena Ave, BR-30130100 Belo Horizonte, MG, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPEMIG: CDS-APQ-02373-17
dc.format.extent359-367
dc.identifierhttp://dx.doi.org/10.3892/ol.2019.11095
dc.identifier.citationOncology Letters. Athens: Spandidos Publ Ltd, v. 19, n. 1, p. 359-367, 2020.
dc.identifier.doi10.3892/ol.2019.11095
dc.identifier.issn1792-1074
dc.identifier.urihttp://hdl.handle.net/11449/196508
dc.identifier.wosWOS:000508369500040
dc.language.isoeng
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofOncology Letters
dc.sourceWeb of Science
dc.subjectovarian cancer
dc.subjectprognosis
dc.subjectresistance to chemotherapy
dc.subjectheat shock proteins
dc.subjectgene expression
dc.titleHSPA1A, HSPA1L and TRAP1 heat shock genes may be associated with prognosis in ovarian epithelial canceren
dc.typeArtigo
dcterms.rightsHolderSpandidos Publ Ltd

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