Innovative strategy for in-office tooth bleaching using violet LED and biopolymers as H2O2 catalysts

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dc.contributor.authorMartins, Beatriz Voss [UNESP]
dc.contributor.authorDias, Marlon Ferreira [UNESP]
dc.contributor.authorde Oliveira Ribeiro, Rafael Antônio [UNESP]
dc.contributor.authorLeite, Maria Luísa de Alencar e Silva [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-03-02T00:28:51Z
dc.date.available2023-03-02T00:28:51Z
dc.date.issued2022-06-01
dc.description.abstractObjective: To assess the influence of coating the enamel with a nanofiber scaffold (NS) and a polymeric catalyst primer (PCP) on the esthetic efficacy, degradation kinetics of hydrogen peroxide (H2O2), and trans-amelodentinal cytotoxicity of bleaching gels subjected or not to violet-LED irradiation. Methodology: The following groups were established (n = 8): G1- No treatment (negative control); G2- NS+PCP; G3- LED; G4- NS+PCP+LED; G5- 35% H2O2 (positive control); G6- NS+PCP+35% H2O2+LED; G7- 20% H2O2; G8- NS+PCP+20% H2O2+LED; G9- 10% H2O2; G10- NS+PCP+10% H2O2+LED. For esthetic efficacy analysis, enamel/dentin discs were stained and exposed for 45 min to the bleaching protocols. To assess the cytotoxicity, the stained enamel/dentin discs were adapted to artificial pulp chambers, and the extracts (culture medium + components diffused through the discs) were collected and applied to MDPC-23 cells, which had their viability, oxidative stress, and morphology (SEM) evaluated. The amount of H2O2 diffused and hydroxyl radical (OH•) production were also determined (two-way ANOVA/Tukey/paired Student t-test; p < 0.05). Results: G6 had the highest esthetic efficacy compared to the other groups (p < 0.05). Besides the esthetic efficacy similar to conventional in-office bleaching (G5; p > 0.05), G10 also showed the lowest toxic effect and oxidative stress to MDPC-23 cells compared to all bleached groups (p < 0.05). Conclusion: Coating the enamel with a nanofiber scaffold and a polymeric catalyst primer, followed by the application of 10%, 20%, or 35% H2O2 bleaching gels irradiated with a violet LED, stimulates H2O2 degradation, increasing esthetic efficacy and reducing the trans-amelodentinal toxicity of the treatment.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry
dc.description.affiliationUnespDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2020/08882–6
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2022.102886
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 38.
dc.identifier.doi10.1016/j.pdpdt.2022.102886
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-85129668470
dc.identifier.urihttp://hdl.handle.net/11449/241813
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.sourceScopus
dc.subjectCytotoxicity
dc.subjectOdontoblasts
dc.subjectTooth bleaching
dc.subjectviolet
dc.titleInnovative strategy for in-office tooth bleaching using violet LED and biopolymers as H2O2 catalystsen
dc.typeArtigo

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