Serological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard Brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests

dc.contributor.authorNardi Junior, G. [UNESP]
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.authorJorge, Andre Mendes [UNESP]
dc.contributor.authorMegid, Jane [UNESP]
dc.contributor.authorSilva, L. M. P.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Biol
dc.date.accessioned2013-09-30T18:26:22Z
dc.date.accessioned2014-05-20T13:41:14Z
dc.date.available2013-09-30T18:26:22Z
dc.date.available2014-05-20T13:41:14Z
dc.date.issued2012-03-01
dc.description.abstractThe serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using Brucella abortus strain 19 (S19) were evaluated by rose bengal (RBT), 2-mercaptoethanol (2ME) and complement fixation (CFT) tests. The serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. No animal showed reaction in day zero. In 15th day above 95% of animals revealed reaction in all tests. All the animals presented absence of reactions in CFT, RBT and 2ME tests at 270, 300 and 360 days after vaccination, respectively. Our finding highlighted early response in CFT compared than other conventional agglutination tests. None of animals presented oscillation of titers or reactions in any test after 360 day of study, which enables the use of these tests after this period without interference of antibodies from S19 vaccine origin between 3 and 8 months in buffalo heifers. (c) 2011 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Sch Vet Med, Dept Vet Hyg & Publ Hlth, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Sch Vet Med, Dept Anim Prod, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationInst Biol, Ctr Pesquisa & Desenvolvimento Sanidade Anim, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Vet Med, Dept Vet Hyg & Publ Hlth, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Vet Med, Dept Anim Prod, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent158-161
dc.identifierhttp://dx.doi.org/10.1016/j.biologicals.2011.09.016
dc.identifier.citationBiologicals. London: Academic Press Ltd- Elsevier B.V. Ltd, v. 40, n. 2, p. 158-161, 2012.
dc.identifier.doi10.1016/j.biologicals.2011.09.016
dc.identifier.issn1045-1056
dc.identifier.lattes2209124317273797
dc.identifier.lattes8878595413452908
dc.identifier.urihttp://hdl.handle.net/11449/14287
dc.identifier.wosWOS:000302426400008
dc.identifier.wosWOS:000331980300192
dc.language.isoeng
dc.publisherAcademic Press Ltd Elsevier B.V. Ltd
dc.relation.ispartofBiologicals
dc.relation.ispartofjcr1.872
dc.relation.ispartofsjr0,636
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBrucellosisen
dc.subjectBrucella abortusen
dc.subjectBuffaloen
dc.subjectVaccinationen
dc.subjectStrain 19en
dc.subjectSerodiagnosisen
dc.titleSerological profile of buffalo (Bubalus bubalis) female calves vaccinated with standard Brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation testsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Ltd- Elsevier B.V. Ltd
unesp.author.lattes2209124317273797
unesp.author.lattes8878595413452908[3]
unesp.author.orcid0000-0002-9079-198X[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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