Protein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluation

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dc.contributor.authorAraújo, Tamara Teodoro
dc.contributor.authorCarvalho, Thamyris Souza
dc.contributor.authorDionizio, Aline
dc.contributor.authorDebortolli, Ana Luiza Bogaz
dc.contributor.authorVentura, Talita Mendes Oliveira
dc.contributor.authorSouza, Beatriz Martines
dc.contributor.authorFeitosa, Chelsea Maria Vilas Boas
dc.contributor.authorBarbosa, Heloisa Aparecida Pereira
dc.contributor.authorRibeiro, Caroline Fernanda da Silva
dc.contributor.authorMartini, Tatiana
dc.contributor.authorTaira, Even
dc.contributor.authorShibao, Priscila Yumi Tanaka
dc.contributor.authorHenrique-Silva, Flavio
dc.contributor.authorMarchetto, Reinaldo [UNESP]
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSão Carlos Federal University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:36:23Z
dc.date.available2022-04-29T08:36:23Z
dc.date.issued2022-01-01
dc.description.abstractObjective: To study the proteomic alterations in the initial AEP after rinsing with CaneCPI-5, StN15 or Hb or their combination. Materials and methods: In five crossover phases, after prophylaxis, 10 volunteers in 5 consecutive days, rinsed (10 mL, 1 min) with the following solutions: deionized water (H2O- negative control- 1), 0.1 mg/mL CaneCPI-5 (2), 1.88×10−5 M StN15 (3), 1.0 mg/mL Hb (4) or their combination (5). The AEP formed after 3 min was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. Results: Rinsing with the proteins/peptide increased the amounts of proteins in the AEP. The total numbers of proteins identified after rinsing with CaneCPI-5, StN15, Hb or their combination versus water, were 131, 167, 148 and 142, respectively. The treatment with the proteins/peptide or their combination increased proteins that bind calcium, phosphate and interact with distinct proteins, as well as proteins with antimicrobial and acid-resistant properties, such as, Cornifin-B (7.7, 12.6, and 4.3-fold for CaneCPI-5, StN15 and Hb, respectively), isoforms of Cystatin (2.2–2.4-fold for CaneCPI-5 and StN15), Proline-rich-protein 4 (4.3-fold; StN15), Histatin-1 (2.8-fold; StN15) and Hemoglobin (7.7–25-fold for Hb and Combination). Immunoglobulin, Keratin and Histone were exclusively identified upon treatment with the proteins/peptide, alone or combined. Conclusion: Rinsing with proteins/peptide, alone or combined, increased protective proteins in the initial AEP. Clinical Relevance: Our results suggest that rinsing with the proteins/peptide or their combination increases the proteins capable of enhancing the protective function of the basal layer of AEP.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo
dc.description.affiliationDepartment of Genetics and Evolution São Carlos Federal University
dc.description.affiliationDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biochemistry and Technology Institute of Chemistry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2021.103874
dc.identifier.citationJournal of Dentistry, v. 116.
dc.identifier.doi10.1016/j.jdent.2021.103874
dc.identifier.issn0300-5712
dc.identifier.scopus2-s2.0-85119117048
dc.identifier.urihttp://hdl.handle.net/11449/229891
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.sourceScopus
dc.subjectAcquired enamel pellicle
dc.subjectCaneCPI-5
dc.subjectHemoglobin
dc.subjectProteomics
dc.subjectStatherin
dc.titleProtein-based engineering of the initial acquired enamel pellicle in vivo: Proteomic evaluationen
dc.typeArtigo

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