Adherence and intracellular parasitism of Paracoccidioides brasiliensis in Vero cells

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dc.contributor.authorHanna, S. A.
dc.contributor.authorda Silva, JLM
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:37Z
dc.date.available2014-05-20T13:24:37Z
dc.date.issued2000-07-01
dc.description.abstractParacoccidioides brasiliensis is a dimorphic fungus known to produce invasive systemic disease in humans. The 43-kDa glycoprotein of P, brasiliensis is the major diagnostic antigen of paracoccidioidomycosis and may act as a virulence factor, since it is a receptor for laminin. Very little is known about early interact-ions between this fungus and the host cells, so we developed in vitro a model system employing cultured mammalian cells (Vero cells), in order to investigate the factors and virulence mechanisms of P. brasiliensis related to the adhesion and invasion process. We found that there is a permanent interaction after 30 min of contact between the fungus and the cells. The yeasts multiply in the cells for between 5 and 24 h. Different strains of P, brasiliensis were compared, and strain 18 thigh virulence) was the most strongly adherent, followed by strain 113 (virulent), 265 (considered of low virulence) and 113M(mutant obtained by ultraviolet radiation, deficient in gp43). P. brasiliensis adhered to the epithelial cells by a narrow tube, while depressions were noticed in the cell surface, suggesting an active cavitation process. An inhibition assay was performed and it was verified that anti-gp43 serum and a pool of sera from individuals with paracoccidioidomycosis were able to inhibit the adhesion of P. brasiliensis to the Vero cells. Glycoprotein 43 (gp43) antiserum abolished 85 % of the binding activity of P. brasiliensis. This fungus can also invade the Vero cells, and intraepithelial parasitism could be an escape mechanism in paracoccidioidomycosis. (C) 2000 Editions scientifiques et medicales Elsevier SAS.en
dc.description.affiliationUNESP, Fac Ciências Farmaceut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil
dc.format.extent877-884
dc.identifierhttp://dx.doi.org/10.1016/S1286-4579(00)00390-7
dc.identifier.citationMicrobes and Infection. Paris Cedex 15: Editions Scientifiques Medicales Elsevier, v. 2, n. 8, p. 877-884, 2000.
dc.identifier.doi10.1016/S1286-4579(00)00390-7
dc.identifier.issn1286-4579
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.urihttp://hdl.handle.net/11449/7697
dc.identifier.wosWOS:000088846600003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.relation.ispartofjcr2.924
dc.relation.ispartofsjr1,205
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectParacoccidioides brasiliensispt
dc.subjectadhesionpt
dc.subjectinvasionpt
dc.titleAdherence and intracellular parasitism of Paracoccidioides brasiliensis in Vero cellsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-8059-0826[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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