Development of a real-time PCR method for rapid sexing of human preimplantation embryos

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dc.contributor.authorMartinhago, C. D. [UNESP]
dc.contributor.authorVagnini, L. D.
dc.contributor.authorPetersen, C. G.
dc.contributor.authorMauri, A. L.
dc.contributor.authorBaruffi, R. L.
dc.contributor.authorde Oliveira, R. M.
dc.contributor.authorFranco, J. G. [UNESP]
dc.contributor.institutionCtr Human Reprod Prof Franco Jr
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPaulista Ctr Diag & Res
dc.contributor.institutionRDO Med Diag
dc.date.accessioned2014-05-20T13:35:19Z
dc.date.available2014-05-20T13:35:19Z
dc.date.issued2010-01-01
dc.description.abstractGenes on the X chromosome are known to be responsible for more than 200 hereditary diseases. After IVF, the simple selection of embryo sex before uterine transfer can prevent the occurrence of affected offspring among couples at risk for these genetic disorders. The aim of this investigation was to develop a rapid method of preimplantation genetic diagnosis (PGD) using real-time polymerase chain reaction (PCR) for the sexing of human embryos, and to compare it to the fluorescence in-situ hybridization technique, considered to be the gold standard. After biopsies were obtained from 40 surplus non-viable embryos for transfer, a total of 98 blastomeres were analysed. It was possible to analyse 24 embryos (60%) by both techniques, generating a total of 70 blastomeres (35 per technique), white 28 blastomeres from 16 embryos (40%) were analysed only by real-time PCR. A rapid and safe method was developed in the present study for the sexual diagnosis of a single human cell (blastomere and buccal cell) using the emerging technology of real-time PCR. (C) 2009, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationCtr Human Reprod Prof Franco Jr, CRH, Ribeirao Preto, Brazil
dc.description.affiliationUNESP, Botucatu Med Sch, Doctoral Postgrad Program Gynecol Obstet & Mastol, Botucatu, SP, Brazil
dc.description.affiliationPaulista Ctr Diag & Res, CPDP, Ribeirao Preto, Brazil
dc.description.affiliationRDO Med Diag, São Paulo, Brazil
dc.description.affiliationUNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Botucatu Med Sch, Doctoral Postgrad Program Gynecol Obstet & Mastol, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP, Brazil
dc.format.extent75-82
dc.identifierhttp://dx.doi.org/10.1016/j.rbmo.2009.10.008
dc.identifier.citationReproductive Biomedicine Online. Cambridge: Reproductive Healthcare Ltd, v. 20, n. 1, p. 75-82, 2010.
dc.identifier.doi10.1016/j.rbmo.2009.10.008
dc.identifier.issn1472-6483
dc.identifier.urihttp://hdl.handle.net/11449/12142
dc.identifier.wosWOS:000274958600011
dc.language.isoeng
dc.publisherReproductive Healthcare Ltd
dc.relation.ispartofReproductive BioMedicine Online
dc.relation.ispartofjcr2.967
dc.relation.ispartofsjr1,343
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectfluorescence in-situ hybridization (FISH)en
dc.subjectpreimplantation genetic diagnosis (PGD)en
dc.subjectreal-time quantitative PCRen
dc.subjectsexing human embryosen
dc.subjectsingle cellsen
dc.subjectTaqMan MGB probesen
dc.titleDevelopment of a real-time PCR method for rapid sexing of human preimplantation embryosen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderReproductive Healthcare Ltd
unesp.author.orcid0000-0002-7802-8906[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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