Explant culture: A relevant tool for the study of telocytes

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dc.contributor.authorSanches, Bruno D. A.
dc.contributor.authorMaldarine, Juliana D. S.
dc.contributor.authorTamarindo, Guilherme H.
dc.contributor.authorDa Silva, Alana D. T. [UNESP]
dc.contributor.authorLima, Maria L. D. [UNESP]
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorGóes, Rejane M. [UNESP]
dc.contributor.authorTaboga, Sebastião R. [UNESP]
dc.contributor.authorCarvalho, Hernandes F.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:20:51Z
dc.date.available2020-12-12T02:20:51Z
dc.date.issued2020-01-01
dc.description.abstractTelocytes are cells present in the stroma of various tissues including the prostate. The detection of telocytes is still very much dependent on obtaining ultrastructural data that show the presence of telopodes, which are cytoplasmic projections that alternate between dilated regions, the podoms, and thin segments, the podomers. These structures are the distinctive characteristics of the telocytes. Thus, in vitro assays are important for the study of telocytes, which are more easily identified in culture, which also enables the experimental manipulation of these cells. The isolation of telocytes per se does not allow the analysis of the behavior of these cells in relation to other cell types in a given organ. In this sense, in the prostate, explants could be a useful tool for the study of telocytes. The present study obtained prostatic explants and evaluated the influence of recombinant proteins, scattering factor (SCF) and stromal-derived factor 1 (SDF-1), which could impact on the migration of CD34-positive cells. Telocytes migrate out of explants and SDF-1 stimulates the proliferation and formation of telocyte networks in vitro. Telocytes are not smooth muscle cell progenitors in the prostate; on the contrary, they are CD90- and CD44-negative cells and, hence, have limited progenitor capacity. The present study demonstrated that explants are useful tools to elucidate the nature of telocytes and their functions.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biology State University of Campinas (UNICAMP)
dc.description.affiliationDepartment of Biology Laboratory of Microscopy and Microanalysis São Paulo State University (UNESP)
dc.description.affiliationDepartment of Biology Laboratory of Genome Studies São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biology Laboratory of Microscopy and Microanalysis São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biology Laboratory of Genome Studies São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1002/cbin.11446
dc.identifier.citationCell Biology International.
dc.identifier.doi10.1002/cbin.11446
dc.identifier.issn1095-8355
dc.identifier.issn1065-6995
dc.identifier.lattes7991082362671212
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.scopus2-s2.0-85089992728
dc.identifier.urihttp://hdl.handle.net/11449/200970
dc.language.isoeng
dc.relation.ispartofCell Biology International
dc.sourceScopus
dc.subjectc-Kit
dc.subjectCD34
dc.subjectprostate
dc.subjectSCF
dc.subjectSDF-1
dc.subjectsmooth muscle cells
dc.titleExplant culture: A relevant tool for the study of telocytesen
dc.typeArtigo
unesp.author.lattes7991082362671212[6]
unesp.author.orcid0000-0001-6584-6566[3]
unesp.author.orcid0000-0002-0970-4288[8]
unesp.author.orcid0000-0002-3080-9447[9]
unesp.author.orcid0000-0001-5693-6148[6]

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