Amperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladium

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dc.contributor.authorMatos, Renato C.
dc.contributor.authorAugelli, Márcio A. [UNESP]
dc.contributor.authorPedrotti, Jairo J.
dc.contributor.authorLago, Claudimir L.
dc.contributor.authorAngnes, Lúcio
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T18:53:18Z
dc.date.available2022-04-28T18:53:18Z
dc.date.issued1998-01-01
dc.description.abstractA simple and attractive method for quantification of ascorbic acid (AA) in beers, soda, natural juices and commercial vitamin C tablets was achieved by combining flow injection analysis and amperometric detection. An array of gold microelectrodes electrochemically modified by deposition of palladium was employed as working electrode which was almost unaffected by fouling effects. Ascorbic acid was quantified in beverages and vitamin tablets using amperometric differential measurements. This method is based on three steps involving the flow injection of: 1) the sample plus a standard addition of AA, 2) the pure sample, and 3) the enzymatically-treated sample. The enzymatic treatment was carried out with Cucumis sativus tissue, which is a rich source of ascorbate oxidase, at pH 7. The calibration plots for freshly prepared ascorbic acid standards were very linear in the concentration range of 0.18-1.8 mg L-1 with a relative standard deviation (RSD) < 1%, while for real samples the deviations were between 2.7 % to 8.9 %.en
dc.description.affiliationInstituto de Química Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-900-São Paulo, SP
dc.description.affiliationFaculdade Engenharia Guaratingueta UNESP, Guaratinguetá, SP
dc.description.affiliationUnespFaculdade Engenharia Guaratingueta UNESP, Guaratinguetá, SP
dc.format.extent887-890
dc.identifierhttp://dx.doi.org/10.1002/(SICI)1521-4109(199810)10:13<887
dc.identifier.citationElectroanalysis, v. 10, n. 13, p. 887-890, 1998.
dc.identifier.doi10.1002/(SICI)1521-4109(199810)10:13<887
dc.identifier.issn1040-0397
dc.identifier.scopus2-s2.0-0001199067
dc.identifier.urihttp://hdl.handle.net/11449/219003
dc.language.isoeng
dc.relation.ispartofElectroanalysis
dc.sourceScopus
dc.subjectAscorbic acid
dc.subjectBeverages
dc.subjectFlow injection analysis
dc.subjectModified microelectrodes
dc.subjectVitamin C
dc.titleAmperometric Differential Determination of Ascorbic Acid in Beverages and Vitamin C Tablets Using a Flow Cell Containing an Array of Gold Microelectrodes Modified with Palladiumen
dc.typeArtigo

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