DETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS)

Página do item simplificado
dc.contributor.authorCarneiro, Leandro B. [UNESP]
dc.contributor.authorMoraes, Marli L.
dc.contributor.authorRibeiro, Sidney J. L. [UNESP]
dc.contributor.authorBrolo, Alexandre G.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Victoria
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2020-12-11T04:37:23Z
dc.date.available2020-12-11T04:37:23Z
dc.date.issued2019-01-01
dc.description.abstractA bioanalytical method for HIV detection based on surface-enhanced Raman scattering (SERS) is described. The assay targeted the peptide p17-1. This peptide sequence is present in the protein p17, a structural protein essential in the life cycle of the human immunodeficiency retrovirus (HIV). The antibody anti-p17 was immobilized in a planar gold surface using self-assembled techniques. This planar platform was used to capture the target analyte (p17-1 peptide). The peptide detection was carried out using specially designed SERS probes, consisting of gold nanoparticles coated with a Raman reporter molecule (Nile Blue A) and functionalized with anti-p17. This sandwich assay led to the successful detection of the peptide p17-1 at 40.8 ng/mL levels. The sandwich assay described here demonstrated that the SERS technique can rival more common fluorescence methods, such as ELISA, in the detection HIV-type virus.en
dc.description.affiliationUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUniv Victoria, Dept Chem, Victoria, BC, Canada
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencia & Tecnol, BR-12247014 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUniv Victoria, Ctr Adv Mat & Related Technol, Victoria, BC, Canada
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil
dc.description.sponsorshipNSERC (Canada)
dc.description.sponsorshipCFI
dc.description.sponsorshipBCKDF
dc.description.sponsorshipUniversity of Victoria
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1038-1043
dc.identifierhttp://dx.doi.org/10.21577/0100-4042.20170430
dc.identifier.citationQuimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 42, n. 9, p. 1038-1043, 2019.
dc.identifier.doi10.21577/0100-4042.20170430
dc.identifier.fileS0100-40422019004901038.pdf
dc.identifier.issn0100-4042
dc.identifier.scieloS0100-40422019004901038
dc.identifier.urihttp://hdl.handle.net/11449/197571
dc.identifier.wosWOS:000500938600007
dc.language.isoeng
dc.publisherSoc Brasileira Quimica
dc.relation.ispartofQuimica Nova
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectHIV
dc.subjectSERS
dc.subjectpeptide p17-1
dc.subjectSERS probes
dc.subjectSERS Immunoassay
dc.titleDETECTION OF THE PEPTIDE P17-1 (HIV) BY SURFACE ENHANCE RAMAN SCATTERING (SERS)en
dc.typeArtigo
dcterms.rightsHolderSoc Brasileira Quimica

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0100-40422019004901038.pdf
Tamanho:
3.07 MB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Química Inorgânica - IQ