Magnetic 3D cell culture: State of the art and current advances

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dc.contributor.authorCaleffi, Juliana Trindade [UNESP]
dc.contributor.authorAal, Mirian Carolini Esgoti [UNESP]
dc.contributor.authorGallindo, Helena de Oliveira Manacorda [UNESP]
dc.contributor.authorCaxali, Gabriel Henrique [UNESP]
dc.contributor.authorCrulhas, Bruno Pereira
dc.contributor.authorRibeiro, Amanda Oliveira [UNESP]
dc.contributor.authorSouza, Glauco R.
dc.contributor.authorDelella, Flávia Karina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionRemedy Biologics
dc.contributor.institutionUniversity of Texas Health Sciences Center at Houston
dc.date.accessioned2022-05-01T09:31:02Z
dc.date.available2022-05-01T09:31:02Z
dc.date.issued2021-12-01
dc.description.abstractCell culture is an important tool for the understanding of cell biology and behavior. In vitro cultivation has been increasingly indispensable for biomedical, pharmaceutical, and biotechnology research. Nevertheless, with the demand for in vitro experimentation strategies more representative of in vivo conditions, tridimensional (3D) cell culture models have been successfully developed. Although these 3D models are efficient and address critical questions from different research areas, there are considerable differences between the existing techniques regarding both elaboration and cost. In light of this, this review describes the construction of 3D spheroids using magnetization while bringing the most recent updates in this field. Magnetic 3D cell culture consists of magnetizing cells using an assembly of gold and iron oxide nanoparticles cross-linked with poly-L-lysine nanoparticles. Then, 3D culture formation in special plates with the assistance of magnets for levitation or bioprinting. Here, we discuss magnetic 3D cell culture advancements, including tumor microenvironment, tissue reconstruction, blood vessel engineering, toxicology, cytotoxicity, and 3D culture of cardiomyocytes, bronchial and pancreatic cells.en
dc.description.affiliationSão Paulo State University (UNESP) Institute of Biosciences Department of Structural and Functional Biology
dc.description.affiliationRemedy Biologics
dc.description.affiliationUniversity of Texas Health Sciences Center at Houston
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Biosciences Department of Structural and Functional Biology
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2019/01869-7
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2021.120028
dc.identifier.citationLife Sciences, v. 286.
dc.identifier.doi10.1016/j.lfs.2021.120028
dc.identifier.issn1879-0631
dc.identifier.issn0024-3205
dc.identifier.scopus2-s2.0-85116565273
dc.identifier.urihttp://hdl.handle.net/11449/233628
dc.language.isoeng
dc.relation.ispartofLife Sciences
dc.sourceScopus
dc.subjectMagnetic nanoparticles
dc.subjectMagnetic ring formation
dc.subjectMagnetic spheroid formation
dc.subjectTridimensional cell culture
dc.titleMagnetic 3D cell culture: State of the art and current advancesen
dc.typeResenha

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