A fast-multiclass method for the determination of 21 endocrine disruptors in human urine by using vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and LC-MS/MS

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dc.contributor.authorBocato, Mariana Zuccherato
dc.contributor.authorCesila, Cibele Aparecida
dc.contributor.authorLataro, Beatriz Favero
dc.contributor.authorde Oliveira, Anderson Rodrigo Moraes [UNESP]
dc.contributor.authorCampíglia, Andres Dobal
dc.contributor.authorBarbosa Jr, Fernando
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade de Ribeirão Preto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Central Florida
dc.date.accessioned2020-12-12T02:25:20Z
dc.date.available2020-12-12T02:25:20Z
dc.date.issued2020-10-01
dc.description.abstractSimplicity, speed, and reduced cost are essential demands for routine analysis in human biomonitoring studies. Moreover, the availability of higher volumes of human specimens is becoming more restrictive due to ethical controls and to the costs associated with sample transportation and storage. Thus, analytical methods requiring much lower sample volumes associated with simultaneous detection capability (multiclass analysis) are with a very high claim. In this sense, the present approach aimed at the development of a method for preconcentration and simultaneous determination of four classes of endocrine disruptors (seven bisphenols, seven parabens, five benzophenones, and two antimicrobials) in the urine. The approach is based on vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). After optimization of the significant parameters of VADLLME extraction, the proposed procedure showed to be simple, fast, sensitive, requiring only 1.0 mL of urine, 400 μL of organic solvents with a total stirring time of 20 s. Moreover, a variation of inter-day and between-day runs were lower than 10.0% and 11.0%, respectively. Finally, the proposed method was successfully applied to the analysis of 50 urine samples of Brazilian pregnant women to establish reference ranges.en
dc.description.affiliationDepartamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationFaculdade de Ciências Farmacêuticas Universidade de Ribeirão Preto
dc.description.affiliationDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationNational Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry
dc.description.affiliationDepartment of Chemistry University of Central Florida, P.O.Box 25000
dc.description.affiliationUnespNational Institute for Alternative Technologies of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 14/50867–3
dc.description.sponsorshipIdCNPq: 154866/2018–0
dc.description.sponsorshipIdFAPESP: 2014/19914–5
dc.description.sponsorshipIdFAPESP: 2014/50945–4
dc.description.sponsorshipIdFAPESP: 2015/20725–5
dc.description.sponsorshipIdFAPESP: 2018/07534–4
dc.description.sponsorshipIdFAPESP: 2018/24069–3
dc.description.sponsorshipIdCNPq: 309822/2017–3
dc.description.sponsorshipIdCNPq: 422588/2016–4
dc.description.sponsorshipIdCNPq: 465389/2014–7
dc.description.sponsorshipIdCNPq: 465571/2014–0
dc.description.sponsorshipIdCAPES: 88887.370750/2019–00
dc.identifierhttp://dx.doi.org/10.1016/j.envres.2020.109883
dc.identifier.citationEnvironmental Research, v. 189.
dc.identifier.doi10.1016/j.envres.2020.109883
dc.identifier.issn1096-0953
dc.identifier.issn0013-9351
dc.identifier.scopus2-s2.0-85088013818
dc.identifier.urihttp://hdl.handle.net/11449/201149
dc.language.isoeng
dc.relation.ispartofEnvironmental Research
dc.sourceScopus
dc.subjectEndocrine disruptors
dc.subjectHuman urine
dc.subjectLiquid chromatography-tandem mass spectrometry
dc.subjectMulticlass analysis
dc.subjectVortex-assisted dispersive liquid-liquid microextraction
dc.titleA fast-multiclass method for the determination of 21 endocrine disruptors in human urine by using vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and LC-MS/MSen
dc.typeArtigo

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Artigos - Princípios Ativos Naturais e Toxicologia - FCFAR