Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction

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dc.contributor.authorMeireles, Marcelo Vasconcelos [UNESP]
dc.contributor.authorRoberto, LO
dc.contributor.authorRiera, RF
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:07:13Z
dc.date.available2014-05-20T15:07:13Z
dc.date.issued2004-12-01
dc.description.abstractThere are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA extraction using DNAzol followed by PCR can be a useful tool in epidemiological studies, since it provides fast and reliable detection of Eimeria sp. in field samples.en
dc.description.affiliationUniversidade Estadual Paulista
dc.description.affiliationUnespUniversidade Estadual Paulista
dc.format.extent249-252
dc.identifierhttp://dx.doi.org/10.1590/S1516-635X2004000400010
dc.identifier.citationRevista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 4, p. 249-252, 2004.
dc.identifier.doi10.1590/S1516-635X2004000400010
dc.identifier.fileS1516-635X2004000400010.pdf
dc.identifier.issn1516-635X
dc.identifier.lattes0903513897615274
dc.identifier.orcid0000-0003-0063-5172
dc.identifier.scieloS1516-635X2004000400010
dc.identifier.urihttp://hdl.handle.net/11449/26418
dc.language.isoeng
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relation.ispartofRevista Brasileira de Ciência Avícola
dc.relation.ispartofjcr0.463
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectdiagnosisen
dc.subjectEimeria mitisen
dc.subjectEimeria praecoxen
dc.subjectepidemiologyen
dc.subjectpolymerase chain reactionen
dc.titleIdentification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reactionen
dc.typeArtigo
unesp.author.lattes0903513897615274
unesp.author.orcid0000-0003-0063-5172[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA