Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

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dc.contributor.authorCoimbra, John L P
dc.contributor.authorDantas, Graziela de P F
dc.contributor.authorde Andrade, Lídia M
dc.contributor.authorBrener, Marcos R G
dc.contributor.authorViana, Pedro I M
dc.contributor.authorLopes, Roberta A.
dc.contributor.authorO G Gontijo, Daniele
dc.contributor.authorErvilha, Luiz O G
dc.contributor.authorAssis, Mirian Q.
dc.contributor.authorBarcelos, Luciola S.
dc.contributor.authorE Szawka, Raphael
dc.contributor.authorDamasceno, Débora C [UNESP]
dc.contributor.authorMachado-Neves, Mariana
dc.contributor.authorMota, Ana P.
dc.contributor.authorCosta, Guilherme M J
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionMG
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:57:41Z
dc.date.available2023-07-29T13:57:41Z
dc.date.issued2023-06-15
dc.description.abstractThis study aimed to evaluate the gold nanoparticles (AuNPs) animal sterilizing potential after intratesticular injections and long-term adverse reproductive and systemic effects. Adult male Wistar rats were divided into control and gold nanoparticle (AuNPs) groups. The rats received 200 µL of saline or AuNPs solution (16 µg/mL) on experimental days 1 and 7 (ED1 and ED7). After 150 days, the testicular blood flow was measured, and the rats were mated with females. After mating, male animals were euthanized for histological, cellular, and molecular evaluations. The female fertility indices and fetal development were also recorded. The results indicated increased blood flow in the testes of treated animals. Testes from treated rats had histological abnormalities, shorter seminiferous epithelia, and oxidative stress. Although the sperm concentration was lower in the AuNP-treated rats, there were no alterations in sperm morphology. Animals exposed to AuNPs had decreased male fertility indices, and their offspring had lighter and less efficient placentas. Additionally, the anogenital distance was longer in female fetuses. There were no changes in the histology of the kidney and liver, the lipid profile, and the serum levels of LH, testosterone, AST, ALT, ALP, albumin, and creatinine. The primary systemic effect was an increase in MDA levels in the liver and kidney, with only the liver experiencing an increase in CAT activity. In conclusion, AuNPs have a long-term impact on reproduction with very slight alterations in animal health. The development of reproductive biotechnologies that eliminate germ cells or treat local cancers can benefit from using AuNPs.en
dc.description.affiliationLaboratory of Cellular Biology Department of Morphology Institute of Biological Sciences Federal University of Minas Gerais MG
dc.description.affiliationLaboratory of Nanomaterials Nanobiomedical Research Group MG
dc.description.affiliationLaboratory of Endocrinology and Metabolism Department of Physiology and Pharmacology Institute of Biological Sciences Federal University of Minas Gerais MG
dc.description.affiliationDepartment of Physiology and Biophysics Institute of Biological Sciences Federal University of Minas Gerais MG
dc.description.affiliationLaboratory of Animal Reproduction and Toxicology Department of General Biology MG
dc.description.affiliationLaboratory of Experimental Research on Gynecology and Obstetrics Postgraduate Course of Tocogynecology Botucatu Medical School Unesp
dc.description.affiliationClinical Hematology Laboratory Faculty of Pharmacy Biological Sciences Federal University of Minas Gerais MG
dc.description.affiliationUnespLaboratory of Experimental Research on Gynecology and Obstetrics Postgraduate Course of Tocogynecology Botucatu Medical School Unesp
dc.format.extent153543
dc.identifierhttp://dx.doi.org/10.1016/j.tox.2023.153543
dc.identifier.citationToxicology, v. 492, p. 153543-.
dc.identifier.doi10.1016/j.tox.2023.153543
dc.identifier.issn1879-3185
dc.identifier.scopus2-s2.0-85160877707
dc.identifier.urihttp://hdl.handle.net/11449/248932
dc.language.isoeng
dc.relation.ispartofToxicology
dc.sourceScopus
dc.subjectEpididymis
dc.subjectNanotechnology
dc.subjectOxidative stress
dc.subjectPlacenta
dc.subjectSperm
dc.subjectTestis
dc.titleGold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implicationsen
dc.typeArtigo

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