CaPrx, a Coffea arabica gene encoding a putative class III peroxidase induced by root-knot nematode infection

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dc.contributor.authorSeverino, Fabio E. [UNESP]
dc.contributor.authorBrandalise, Marcos [UNESP]
dc.contributor.authorCosta, Carolina S. [UNESP]
dc.contributor.authorWilcken, Silvia R. S. [UNESP]
dc.contributor.authorMaluf, Mirian P.
dc.contributor.authorGoncalves, Wallace
dc.contributor.authorMaia, Ivan de Godoy [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionInstituto Agronômico (IAC)
dc.date.accessioned2014-05-20T13:50:25Z
dc.date.available2014-05-20T13:50:25Z
dc.date.issued2012-08-01
dc.description.abstractClass III peroxidases (Prxs) are enzymes involved in a multitude of physiological and stress-related processes in plants. Here, we report on the characterization of a putative peroxidase-encoding gene from Coffea arabica (CaPrx) that is expressed in early stages of root-knot nematode (RKN) infection. CaPrx showed enhanced expression in coffee roots inoculated with RKN (at 12 h post-inoculation), but no significant difference in expression was observed between susceptible and resistant plants. Assays using transgenic tobacco plants harboring a promoter-beta-glucuronidase (GUS) fusion revealed that the CaPrx promoter was exclusively active in the galls induced by RKN. In cross sections of galls, GUS staining was predominantly localized in giant cells. Up-regulation of GUS expression in roots of transgenic plants following RKN inoculation was observed within 16 h. Moreover, no increase in GUS expression after treatment with jasmonic acid was detected. Altogether, these results point to a putative role of this peroxidase in the general coffee response to RKN infection. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Cafe, Brasilia, DF, Brazil
dc.description.affiliationUNESP, Fac Ciencias Agron, Dept Defesa Fitossanitaria, Botucatu, SP, Brazil
dc.description.affiliationCtr Cafe Alcides Carvalho, Instituto Agronômico de Campinas, Campinas, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciencias Agron, Dept Defesa Fitossanitaria, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 09/54361-9
dc.format.extent35-42
dc.identifierhttp://dx.doi.org/10.1016/j.plantsci.2012.04.012
dc.identifier.citationPlant Science. Clare: Elsevier B.V., v. 191, p. 35-42, 2012.
dc.identifier.doi10.1016/j.plantsci.2012.04.012
dc.identifier.issn0168-9452
dc.identifier.lattes8649222099176162
dc.identifier.urihttp://hdl.handle.net/11449/17997
dc.identifier.wosWOS:000306255800005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPlant Science
dc.relation.ispartofjcr3.712
dc.relation.ispartofsjr1,667
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCoffeeen
dc.subjectPromoteren
dc.subjectPeroxidasesen
dc.subjectNematodeen
dc.subjectTissue-specificityen
dc.subjectGene expressionen
dc.titleCaPrx, a Coffea arabica gene encoding a putative class III peroxidase induced by root-knot nematode infectionen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8649222099176162
unesp.author.orcid0000-0002-9306-0197[4]
unesp.author.orcid0000-0002-3875-8664[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agronômicas, Botucatupt

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