Polycation-based gene therapy: Current knowledge and new perspectives

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dc.contributor.authorTiera, Marcio J. [UNESP]
dc.contributor.authorShi, Qin
dc.contributor.authorWinnik, Françoise M.
dc.contributor.authorFernandes, Julio C.
dc.contributor.institutionUniversité de Montréal
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T22:49:07Z
dc.date.available2022-04-28T22:49:07Z
dc.date.issued2011-08-01
dc.description.abstractAt present, gene transfection insufficient efficiency is a major drawback of non-viral gene therapy. The 2 main types of delivery systems deployed in gene therapy are based on viral or non-viral gene carriers. Several non-viral modalities can transfer foreign genetic material into the human body. To do so, polycation-based gene delivery methods must achieve sufficient efficiency in the transportation of therapeutic genes across various extracellular and intracellular barriers. These barriers include interactions with blood components, vascular endothelial cells and uptake by the reticuloendothelial system. Furthermore, the degradation of therapeutic DNA by serum nucleases is a potential obstacle for functional delivery to target cells. Cationic polymers constitute one of the most promising approaches to the use of viral vectors for gene therapy. A better understanding of the mechanisms by which DNA can escape from endosomes and traffic to enter the nucleus has triggered new strategies of synthesis and has revitalized research into new polycation-based systems. The objective of this review is to address the state of the art in gene therapy with synthetic and natural polycations and the latest advances to improve gene transfer efficiency in cells. © 2011 Bentham Science Publishers Ltd.en
dc.description.affiliationOrthopedic Research Laboratory Hôpital du Sacré-Coeur de Montréal Université de Montréal
dc.description.affiliationDepartamento de Química e Ciências Ambientais UNESP-Universidade Estadual Paulista, São José do Rio Preto
dc.description.affiliationFaculty of Pharmacy and Department of Chemistry Université de Montréal, Montreal, QC
dc.description.affiliationUnespDepartamento de Química e Ciências Ambientais UNESP-Universidade Estadual Paulista, São José do Rio Preto
dc.format.extent288-306
dc.identifierhttp://dx.doi.org/10.2174/156652311796150408
dc.identifier.citationCurrent Gene Therapy, v. 11, n. 4, p. 288-306, 2011.
dc.identifier.doi10.2174/156652311796150408
dc.identifier.issn1566-5232
dc.identifier.issn1875-5631
dc.identifier.scopus2-s2.0-79960022298
dc.identifier.urihttp://hdl.handle.net/11449/226414
dc.language.isoeng
dc.relation.ispartofCurrent Gene Therapy
dc.sourceScopus
dc.subjectDna
dc.subjectGene therapy
dc.subjectNanoparticles
dc.subjectPolycations
dc.subjectPolymers
dc.titlePolycation-based gene therapy: Current knowledge and new perspectivesen
dc.typeArtigo

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