Complete staining of nerve fiber and myoneural junctions with acetylthiocholine and silver

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dc.contributor.authorBoaro, S. N.
dc.contributor.authorGregorio, E. A.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:25:12Z
dc.date.available2014-05-20T15:25:12Z
dc.date.issued1997-09-01
dc.description.abstractWe describe a combined stain for simultaneous demonstration of the preterminal axons and cholinesterase activity at myoneural junctions of mammalian muscles. This technique employs acetylthiocholine iodide as the substrate for cholinesterase activity and silver nitrate impregnation of preterminal axons. The procedure is rapid, simple and uses fresh muscles. Intramuscular nerves, preterminal axons and myoneural junctions are stained simultaneously brown or black with minimal background staining of connective tissue and muscle fibers.en
dc.description.affiliationUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOSCI,DEPT MORPHOL,BR-18618000 BOTUCATU,SP,BRAZIL
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,INST BIOSCI,DEPT MORPHOL,BR-18618000 BOTUCATU,SP,BRAZIL
dc.format.extent240-242
dc.identifierhttp://dx.doi.org/10.3109/10520299709082246
dc.identifier.citationBiotechnic & Histochemistry. Baltimore: Williams & Wilkins, v. 72, n. 5, p. 240-242, 1997.
dc.identifier.doi10.3109/10520299709082246
dc.identifier.issn1052-0295
dc.identifier.urihttp://hdl.handle.net/11449/35649
dc.identifier.wosWOS:A1997YJ75000002
dc.language.isoeng
dc.publisherWilliams & Wilkins
dc.relation.ispartofBiotechnic & Histochemistry
dc.relation.ispartofjcr1.074
dc.relation.ispartofsjr0,384
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectacetylthiocholinept
dc.subjectmyoneural junctionspt
dc.subjectpreterminal axonspt
dc.subjectsilver impregnationpt
dc.titleComplete staining of nerve fiber and myoneural junctions with acetylthiocholine and silveren
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/page/resources/authors
dcterms.rightsHolderWilliams & Wilkins
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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